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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Buwchitin: A Ruminal Peptide with Antimicrobial Potential against Enterococcus faecalis
|
|---|---|
| Published in |
Frontiers in Chemistry, July 2017
|
| DOI | 10.3389/fchem.2017.00051 |
| Pubmed ID | |
| Authors |
Linda B. Oyama, Jean-Adrien Crochet, Joan E. Edwards, Susan E. Girdwood, Alan R. Cookson, Narcis Fernandez-Fuentes, Kai Hilpert, Peter N. Golyshin, Olga V. Golyshina, Florence Privé, Matthias Hess, Hilario C. Mantovani, Christopher J. Creevey, Sharon A. Huws |
| Abstract |
Antimicrobial peptides (AMPs) are gaining popularity as alternatives for treatment of bacterial infections and recent advances in omics technologies provide new platforms for AMP discovery. We sought to determine the antibacterial activity of a novel antimicrobial peptide, buwchitin, against Enterococcus faecalis. Buwchitin was identified from a rumen bacterial metagenome library, cloned, expressed and purified. The antimicrobial activity of the recombinant peptide was assessed using a broth microdilution susceptibility assay to determine the peptide's killing kinetics against selected bacterial strains. The killing mechanism of buwchitin was investigated further by monitoring its ability to cause membrane depolarization (diSC3(5) method) and morphological changes in E. faecalis cells. Transmission electron micrographs of buwchitin treated E. faecalis cells showed intact outer membranes with blebbing, but no major damaging effects and cell morphology changes. Buwchitin had negligible cytotoxicity against defibrinated sheep erythrocytes. Although no significant membrane leakage and depolarization was observed, buwchitin at minimum inhibitory concentration (MIC) was bacteriostatic against E. faecalis cells and inhibited growth in vitro by 70% when compared to untreated cells. These findings suggest that buwchitin, a rumen derived peptide, has potential for antimicrobial activity against E. faecalis. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 5 | 45% |
| Switzerland | 1 | 9% |
| Unknown | 5 | 45% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 55% |
| Scientists | 5 | 45% |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 47 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 23% |
| Researcher | 8 | 17% |
| Student > Master | 5 | 11% |
| Student > Bachelor | 4 | 9% |
| Professor > Associate Professor | 3 | 6% |
| Other | 8 | 17% |
| Unknown | 8 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 30% |
| Biochemistry, Genetics and Molecular Biology | 8 | 17% |
| Immunology and Microbiology | 4 | 9% |
| Medicine and Dentistry | 3 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Other | 6 | 13% |
| Unknown | 10 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2018.
All research outputs
#3,675,990
of 22,986,950 outputs
Outputs from Frontiers in Chemistry
#227
of 6,000 outputs
Outputs of similar age
#65,295
of 312,615 outputs
Outputs of similar age from Frontiers in Chemistry
#3
of 30 outputs
Altmetric has tracked 22,986,950 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 6,000 research outputs from this source. They receive a mean Attention Score of 2.0. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 312,615 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.