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Fusion of MALDI Spectrometric Imaging and Raman Spectroscopic Data for the Analysis of Biological Samples

Overview of attention for article published in Frontiers in Chemistry, July 2018
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Title
Fusion of MALDI Spectrometric Imaging and Raman Spectroscopic Data for the Analysis of Biological Samples
Published in
Frontiers in Chemistry, July 2018
DOI 10.3389/fchem.2018.00257
Pubmed ID
Authors

Oleg Ryabchykov, Juergen Popp, Thomas Bocklitz

Abstract

Despite of a large number of imaging techniques for the characterization of biological samples, no universal one has been reported yet. In this work, a data fusion approach was investigated for combining Raman spectroscopic data with matrix-assisted laser desorption/ionization (MALDI) mass spectrometric data. It betters the image analysis of biological samples because Raman and MALDI information can be complementary to each other. While MALDI spectrometry yields detailed information regarding the lipid content, Raman spectroscopy provides valuable information about the overall chemical composition of the sample. The combination of Raman spectroscopic and MALDI spectrometric imaging data helps distinguishing different regions within the sample with a higher precision than would be possible by using either technique. We demonstrate that a data weighting step within the data fusion is necessary to reveal additional spectral features. The selected weighting approach was evaluated by examining the proportions of variance within the data explained by the first principal components of a principal component analysis (PCA) and visualizing the PCA results for each data type and combined data. In summary, the presented data fusion approach provides a concrete guideline on how to combine Raman spectroscopic and MALDI spectrometric imaging data for biological analysis.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 64 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 30%
Student > Bachelor 8 13%
Researcher 4 6%
Other 3 5%
Student > Doctoral Student 3 5%
Other 5 8%
Unknown 22 34%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 13%
Chemistry 8 13%
Physics and Astronomy 7 11%
Engineering 4 6%
Agricultural and Biological Sciences 2 3%
Other 14 22%
Unknown 21 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 August 2018.
All research outputs
#14,417,376
of 23,090,520 outputs
Outputs from Frontiers in Chemistry
#1,063
of 6,038 outputs
Outputs of similar age
#185,221
of 326,751 outputs
Outputs of similar age from Frontiers in Chemistry
#49
of 182 outputs
Altmetric has tracked 23,090,520 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,038 research outputs from this source. They receive a mean Attention Score of 2.1. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 326,751 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 182 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.