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Transposon mutagenesis as an approach to improved understanding of Borrelia pathogenesis and biology

Overview of attention for article published in Frontiers in Cellular and Infection Microbiology, January 2014
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3 X users

Citations

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36 Dimensions

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Title
Transposon mutagenesis as an approach to improved understanding of Borrelia pathogenesis and biology
Published in
Frontiers in Cellular and Infection Microbiology, January 2014
DOI 10.3389/fcimb.2014.00063
Pubmed ID
Authors

Tao Lin, Erin B. Troy, Linden T. Hu, Lihui Gao, Steven J. Norris

Abstract

Transposon insertion provides a method for near-random mutation of bacterial genomes, and has been utilized extensively for the study of bacterial pathogenesis and biology. This approach is particularly useful for organisms that are relatively refractory to genetic manipulation, including Lyme disease Borrelia. In this review, progress to date in the application of transposon mutagenesis to the study of Borrelia burgdorferi is reported. An effective Himar1-based transposon vector has been developed and used to acquire a sequence-defined library of nearly 4500 mutants in the infectious, moderately transformable B. burgdorferi B31 derivative 5A18NP1. Analysis of these transposon mutants using signature-tagged mutagenesis (STM) and Tn-seq approaches has begun to yield valuable information regarding the genes important in the pathogenesis and biology of this organism.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 99 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Russia 2 2%
New Zealand 1 1%
United Kingdom 1 1%
Argentina 1 1%
Mexico 1 1%
Unknown 93 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 18 18%
Student > Bachelor 17 17%
Student > Master 15 15%
Researcher 12 12%
Student > Doctoral Student 7 7%
Other 11 11%
Unknown 19 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 29 29%
Biochemistry, Genetics and Molecular Biology 21 21%
Immunology and Microbiology 9 9%
Medicine and Dentistry 5 5%
Chemistry 4 4%
Other 9 9%
Unknown 22 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 June 2020.
All research outputs
#14,781,727
of 22,757,090 outputs
Outputs from Frontiers in Cellular and Infection Microbiology
#3,146
of 6,348 outputs
Outputs of similar age
#183,089
of 305,260 outputs
Outputs of similar age from Frontiers in Cellular and Infection Microbiology
#12
of 28 outputs
Altmetric has tracked 22,757,090 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,348 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,260 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.