Title |
Transposon mutagenesis as an approach to improved understanding of Borrelia pathogenesis and biology
|
---|---|
Published in |
Frontiers in Cellular and Infection Microbiology, January 2014
|
DOI | 10.3389/fcimb.2014.00063 |
Pubmed ID | |
Authors |
Tao Lin, Erin B. Troy, Linden T. Hu, Lihui Gao, Steven J. Norris |
Abstract |
Transposon insertion provides a method for near-random mutation of bacterial genomes, and has been utilized extensively for the study of bacterial pathogenesis and biology. This approach is particularly useful for organisms that are relatively refractory to genetic manipulation, including Lyme disease Borrelia. In this review, progress to date in the application of transposon mutagenesis to the study of Borrelia burgdorferi is reported. An effective Himar1-based transposon vector has been developed and used to acquire a sequence-defined library of nearly 4500 mutants in the infectious, moderately transformable B. burgdorferi B31 derivative 5A18NP1. Analysis of these transposon mutants using signature-tagged mutagenesis (STM) and Tn-seq approaches has begun to yield valuable information regarding the genes important in the pathogenesis and biology of this organism. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 33% |
Members of the public | 1 | 33% |
Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Russia | 2 | 2% |
New Zealand | 1 | 1% |
United Kingdom | 1 | 1% |
Argentina | 1 | 1% |
Mexico | 1 | 1% |
Unknown | 93 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 18 | 18% |
Student > Bachelor | 17 | 17% |
Student > Master | 15 | 15% |
Researcher | 12 | 12% |
Student > Doctoral Student | 7 | 7% |
Other | 11 | 11% |
Unknown | 19 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 29 | 29% |
Biochemistry, Genetics and Molecular Biology | 21 | 21% |
Immunology and Microbiology | 9 | 9% |
Medicine and Dentistry | 5 | 5% |
Chemistry | 4 | 4% |
Other | 9 | 9% |
Unknown | 22 | 22% |