Title |
Challenges in the culture-independent analysis of oral and respiratory samples from intubated patients
|
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Published in |
Frontiers in Cellular and Infection Microbiology, May 2014
|
DOI | 10.3389/fcimb.2014.00065 |
Pubmed ID | |
Authors |
Vladimir Lazarevic, Nadia Gaïa, Stéphane Emonet, Myriam Girard, Gesuele Renzi, Lena Despres, Hannah Wozniak, Javier Yugueros Marcos, Jean-Baptiste Veyrieras, Sonia Chatellier, Alex van Belkum, Jérôme Pugin, Jacques Schrenzel |
Abstract |
The spread of microorganisms in hospitals is an important public health threat, and yet few studies have assessed how human microbial communities (microbiota) evolve in the hospital setting. Studies conducted so far have mainly focused on a limited number of bacterial species, mostly pathogenic ones and primarily during outbreaks. We explored the bacterial community diversity of the microbiota from oral and respiratory samples of intubated patients hospitalized in the intensive care unit and we discuss the technical challenges that may arise while using culture-independent approaches to study these types of samples. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | 3% |
Germany | 1 | 1% |
Thailand | 1 | 1% |
Unknown | 68 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 17 | 24% |
Student > Ph. D. Student | 16 | 22% |
Student > Master | 11 | 15% |
Student > Bachelor | 6 | 8% |
Student > Doctoral Student | 4 | 6% |
Other | 11 | 15% |
Unknown | 7 | 10% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 17 | 24% |
Immunology and Microbiology | 11 | 15% |
Biochemistry, Genetics and Molecular Biology | 5 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 4% |
Other | 8 | 11% |
Unknown | 9 | 13% |