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Mendeley readers
Attention Score in Context
| Title |
Clinical and Genomic Analysis of Liver Abscess-Causing Klebsiella pneumoniae Identifies New Liver Abscess-Associated Virulence Genes
|
|---|---|
| Published in |
Frontiers in Cellular and Infection Microbiology, November 2016
|
| DOI | 10.3389/fcimb.2016.00165 |
| Pubmed ID | |
| Authors |
Meiping Ye, Jianfei Tu, Jianping Jiang, Yingmin Bi, Weibo You, Yanliang Zhang, Jianmin Ren, Taohui Zhu, Zhuo Cao, Zuochun Yu, Chuxiao Shao, Zhen Shen, Baixing Ding, Jinyi Yuan, Xu Zhao, Qinglan Guo, Xiaogang Xu, Jinwei Huang, Minggui Wang |
| Abstract |
Hypervirulent variants of Klebsiella pneumoniae (hvKp) that cause invasive community-acquired pyogenic liver abscess (PLA) have emerged globally. Little is known about the virulence determinants associated with hvKp, except for the virulence genes rmpA/A2 and siderophores (iroBCD/iucABCD) carried by the pK2044-like large virulence plasmid. Here, we collected most recent clinical isolates of hvKp from PLA samples in China, and performed clinical, molecular, and genomic sequencing analyses. We found that 90.9% (40/44) of the pathogens causing PLA were K. pneumoniae. Among the 40 LA-Kp, K1 (62.5%), and K2 (17.5%) were the dominant serotypes, and ST23 (47.5%) was the major sequence type. S1-PFGE analyses demonstrated that although 77.5% (31/40) of the LA-Kp isolates harbored a single large virulence plasmid varied in size, 5 (12.5%) isolates had no plasmid and 4 (10%) had two or three plasmids. Whole genome sequencing and comparative analysis of 3 LA-Kp and 3 non-LA-Kp identified 133 genes present only in LA-Kp. Further, large scale screening of the 133 genes in 45 LA-Kp and 103 non-LA-Kp genome sequences from public databases identified 30 genes that were highly associated with LA-Kp, including iroBCD, iucABCD and rmpA/A2 and 21 new genes. Then, these 21 new genes were analyzed in 40 LA-Kp and 86 non-LA-Kp clinical isolates collected in this study by PCR, showing that new genes were present 80-100% among LA-Kp isolates while 2-11% in K. pneumoniae isolates from sputum and urine. Several of the 21 genes have been proposed as virulence factors in other bacteria, such as the gene encoding SAM-dependent methyltransferase and pagO which protects bacteria from phagocytosis. Taken together, these genes are likely new virulence factors contributing to the hypervirulence phenotype of hvKp, and may deepen our understanding of virulence mechanism of hvKp. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Switzerland | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| New Zealand | 1 | 1% |
| Unknown | 86 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 13% |
| Student > Ph. D. Student | 9 | 10% |
| Student > Master | 9 | 10% |
| Student > Bachelor | 8 | 9% |
| Student > Doctoral Student | 4 | 5% |
| Other | 11 | 13% |
| Unknown | 35 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 16 | 18% |
| Immunology and Microbiology | 15 | 17% |
| Biochemistry, Genetics and Molecular Biology | 12 | 14% |
| Agricultural and Biological Sciences | 5 | 6% |
| Environmental Science | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 37 | 43% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 December 2016.
All research outputs
#20,219,839
of 25,711,518 outputs
Outputs from Frontiers in Cellular and Infection Microbiology
#4,930
of 8,236 outputs
Outputs of similar age
#300,507
of 419,056 outputs
Outputs of similar age from Frontiers in Cellular and Infection Microbiology
#35
of 77 outputs
Altmetric has tracked 25,711,518 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,236 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 419,056 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 24th percentile – i.e., 24% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 77 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.