You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
Interaction of Bacteroides fragilis Toxin with Outer Membrane Vesicles Reveals New Mechanism of Its Secretion and Delivery
|
|---|---|
| Published in |
Frontiers in Cellular and Infection Microbiology, January 2017
|
| DOI | 10.3389/fcimb.2017.00002 |
| Pubmed ID | |
| Authors |
Natalya B. Zakharzhevskaya, Vladimir B. Tsvetkov, Anna A. Vanyushkina, Anna M. Varizhuk, Daria V. Rakitina, Victor V. Podgorsky, Innokentii E. Vishnyakov, Daria D. Kharlampieva, Valentin A. Manuvera, Fedor V. Lisitsyn, Elena A. Gushina, Vassili N. Lazarev, Vadim M. Govorun |
| Abstract |
The only recognized virulence factor of enterotoxigenic Bacteroides fragilis (ETBF) that accompanies bloodstream infections is the zinc-dependent non-lethal metalloprotease B. fragilis toxin (BFT). The isolated toxin stimulates intestinal secretion, resulting in epithelial damage and necrosis. Numerous publications have focused on the interrelation of BFT with intestinal inflammation and colorectal neoplasia, but nothing is known about the mechanism of its secretion and delivery to host cells. However, recent studies of gram-negative bacteria have shown that outer membrane vesicles (OMVs) could be an essential mechanism for the spread of a large number of virulence factors. Here, we show for the first time that BFT is not a freely secreted protease but is associated with OMVs. Our findings indicate that only outer surface-exposed BFT causes epithelial cell contact disruption. According to our in silico models confirmed by Trp quenching assay and NMR, BFT has special interactions with outer membrane components such as phospholipids and is secreted during vesicle formation. Moreover, the strong cooperation of BFT with polysaccharides is similar to the behavior of lectins. Understanding the molecular mechanisms of BFT secretion provides new perspectives for investigating intestinal inflammation pathogenesis and its prevention. |
X Demographics
The data shown below were collected from the profiles of 10 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 30% |
| France | 2 | 20% |
| Switzerland | 1 | 10% |
| Chile | 1 | 10% |
| Canada | 1 | 10% |
| Unknown | 2 | 20% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 7 | 70% |
| Scientists | 3 | 30% |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 67 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 22% |
| Researcher | 11 | 16% |
| Student > Bachelor | 10 | 15% |
| Student > Doctoral Student | 3 | 4% |
| Student > Postgraduate | 3 | 4% |
| Other | 8 | 12% |
| Unknown | 17 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 24 | 36% |
| Agricultural and Biological Sciences | 9 | 13% |
| Immunology and Microbiology | 6 | 9% |
| Medicine and Dentistry | 2 | 3% |
| Chemistry | 2 | 3% |
| Other | 4 | 6% |
| Unknown | 20 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 June 2018.
All research outputs
#6,480,451
of 25,380,192 outputs
Outputs from Frontiers in Cellular and Infection Microbiology
#1,266
of 8,040 outputs
Outputs of similar age
#107,587
of 420,845 outputs
Outputs of similar age from Frontiers in Cellular and Infection Microbiology
#14
of 103 outputs
Altmetric has tracked 25,380,192 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 8,040 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.8. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,845 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 103 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.