Title |
HmsC Controls Yersinia pestis Biofilm Formation in Response to Redox Environment
|
---|---|
Published in |
Frontiers in Cellular and Infection Microbiology, August 2017
|
DOI | 10.3389/fcimb.2017.00355 |
Pubmed ID | |
Authors |
Gai-Xian Ren, Xiao-Peng Guo, Yi-Cheng Sun |
Abstract |
Yersinia pestis biofilm formation, controlled by intracellular levels of the second messenger molecule cyclic diguanylate (c-di-GMP), is important for blockage-dependent plague transmission from fleas to mammals. HmsCDE is a tripartite signaling system that modulates intracellular c-di-GMP levels to regulate biofilm formation in Y. pestis. Previously, we found that Y. pestis biofilm formation is stimulated in reducing environments in an hmsCDE-dependent manner. However, the mechanism by which HmsCDE senses the redox state remains elusive. Using a dsbA mutant and the addition of Cu(2+) to simulate reducing and oxidizing periplasmic environments, we found that HmsC protein levels are decreased and the HmsC-HmsD protein-protein interaction is weakened in a reducing environment. In addition, we revealed that intraprotein disulphide bonds are critical for HmsC since breakage lowers protein stability and diminishes the interaction with HmsD. Our results suggest that HmsC might play a major role in sensing the environmental changes. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 20% |
Student > Master | 2 | 13% |
Student > Doctoral Student | 2 | 13% |
Librarian | 1 | 7% |
Researcher | 1 | 7% |
Other | 1 | 7% |
Unknown | 5 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 3 | 20% |
Biochemistry, Genetics and Molecular Biology | 3 | 20% |
Veterinary Science and Veterinary Medicine | 1 | 7% |
Arts and Humanities | 1 | 7% |
Agricultural and Biological Sciences | 1 | 7% |
Other | 1 | 7% |
Unknown | 5 | 33% |