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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Vaccine-Mediated Mechanisms Controlling Replication of Francisella tularensis in Human Peripheral Blood Mononuclear Cells Using a Co-culture System
|
|---|---|
| Published in |
Frontiers in Cellular and Infection Microbiology, February 2018
|
| DOI | 10.3389/fcimb.2018.00027 |
| Pubmed ID | |
| Authors |
Kjell Eneslätt, Igor Golovliov, Patrik Rydén, Anders Sjöstedt |
| Abstract |
Cell-mediated immunity (CMI) is normally required for efficient protection against intracellular infections, however, identification of correlates is challenging and they are generally lacking.Francisella tularensisis a highly virulent, facultative intracellular bacterium and CMI is critically required for protection against the pathogen, but how this is effectuated in humans is poorly understood. To understand the protective mechanisms, we established anin vitroco-culture assay to identify how control of infection ofF. tularensisis accomplished by human cells and hypothesized that the model will mimicin vivoimmune mechanisms. Non-adherent peripheral blood mononuclear cells (PBMCs) were expanded with antigen and added to cultures with adherent PBMC infected with the human vaccine strain, LVS, or the highly virulent SCHU S4 strain. Intracellular numbers ofF. tularensiswas followed for 72 h and secreted and intracellular cytokines were analyzed. Addition of PBMC expanded from naïve individuals, i.e., those with no record of immunization toF. tularensis, generally resulted in little or no control of intracellular bacterial growth, whereas addition of PBMC from a majority ofF. tularensis-immune individuals executed static and sometimes cidal effects on intracellular bacteria. Regardless of infecting strain, statistical differences between the two groups were significant,P< 0.05. Secretion of 11 cytokines was analyzed after 72 h of infection and significant differences with regard to secretion of IFN-γ, TNF, and MIP-1β was observed between immune and naïve individuals for LVS-infected cultures. Also, in LVS-infected cultures, CD4 T cells from vaccinees, but not CD8 T cells, showed significantly higher expression of IFN-γ, MIP-1β, TNF, and CD107a than cells from naïve individuals. The co-culture system appears to identify correlates of immunity that are relevant for the understanding of mechanisms of the protective host immunity toF. tularensis. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 18% |
| Other | 1 | 9% |
| Student > Doctoral Student | 1 | 9% |
| Lecturer | 1 | 9% |
| Student > Bachelor | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 4 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 2 | 18% |
| Nursing and Health Professions | 1 | 9% |
| Veterinary Science and Veterinary Medicine | 1 | 9% |
| Psychology | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 5 | 45% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 February 2018.
All research outputs
#17,929,042
of 23,020,670 outputs
Outputs from Frontiers in Cellular and Infection Microbiology
#4,173
of 6,510 outputs
Outputs of similar age
#308,282
of 437,836 outputs
Outputs of similar age from Frontiers in Cellular and Infection Microbiology
#86
of 126 outputs
Altmetric has tracked 23,020,670 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,510 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 437,836 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 126 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.