Title |
The Staphylococcus aureus Extracellular Adherence Protein Eap Is a DNA Binding Protein Capable of Blocking Neutrophil Extracellular Trap Formation
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Published in |
Frontiers in Cellular and Infection Microbiology, July 2018
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DOI | 10.3389/fcimb.2018.00235 |
Pubmed ID | |
Authors |
Janina Eisenbeis, Mona Saffarzadeh, Henrik Peisker, Philipp Jung, Nicolas Thewes, Klaus T. Preissner, Mathias Herrmann, Virginie Molle, Brian V. Geisbrecht, Karin Jacobs, Markus Bischoff |
Abstract |
The extracellular adherence protein (Eap) of Staphylococcus aureus is a secreted protein known to exert a number of adhesive and immunomodulatory properties. Here we describe the intrinsic DNA binding activity of this multifunctional secretory factor. By using atomic force microscopy, we provide evidence that Eap can bind and aggregate DNA. While the origin of the DNA substrate (e.g., eukaryotic, bacterial, phage, and artificial DNA) seems to not be of major importance, the DNA structure (e.g., linear or circular) plays a critical role with respect to the ability of Eap to bind and condense DNA. Further functional assays corroborated the nature of Eap as a DNA binding protein, since Eap suppressed the formation of "neutrophil extracellular traps" (NETs), composed of DNA-histone scaffolds, which are thought to function as a neutrophil-mediated extracellular trapping mechanism. The DNA binding and aggregation activity of Eap may thereby protect S. aureus against a specific anti-microbial defense reaction from the host. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 65 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 11 | 17% |
Student > Bachelor | 11 | 17% |
Student > Master | 8 | 12% |
Student > Doctoral Student | 4 | 6% |
Researcher | 4 | 6% |
Other | 9 | 14% |
Unknown | 18 | 28% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 14 | 22% |
Agricultural and Biological Sciences | 10 | 15% |
Medicine and Dentistry | 9 | 14% |
Biochemistry, Genetics and Molecular Biology | 6 | 9% |
Veterinary Science and Veterinary Medicine | 4 | 6% |
Other | 4 | 6% |
Unknown | 18 | 28% |