Title |
Biophysical Detection of Diversity and Bias in GPCR Function
|
---|---|
Published in |
Frontiers in endocrinology, March 2014
|
DOI | 10.3389/fendo.2014.00026 |
Pubmed ID | |
Authors |
Werner C. Jaeger, Stephen P. Armstrong, Stephen J. Hill, Kevin D. G. Pfleger |
Abstract |
Guanine nucleotide binding protein (G protein)-coupled receptors (GPCRs) function in complexes with a range of molecules and proteins including ligands, G proteins, arrestins, ubiquitin, and other receptors. Elements of these complexes may interact constitutively or dynamically, dependent upon factors such as ligand binding, phosphorylation, and dephosphorylation. They may also be allosterically modulated by other proteins in a manner that changes temporally and spatially within the cell. Elucidating how these complexes function has been greatly enhanced by biophysical technologies that are able to monitor proximity and/or binding, often in real time and in live cells. These include resonance energy transfer approaches such as bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET). Furthermore, the use of fluorescent ligands has enabled novel insights into allosteric interactions between GPCRs. Consequently, biophysical approaches are helping to unlock the amazing diversity and bias in G protein-coupled receptor signaling. |
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Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
Hungary | 1 | 33% |
Switzerland | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 1 | 1% |
Switzerland | 1 | 1% |
Netherlands | 1 | 1% |
Indonesia | 1 | 1% |
Czechia | 1 | 1% |
United Kingdom | 1 | 1% |
Denmark | 1 | 1% |
Spain | 1 | 1% |
Unknown | 63 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 18 | 25% |
Researcher | 12 | 17% |
Student > Master | 8 | 11% |
Professor > Associate Professor | 5 | 7% |
Student > Bachelor | 5 | 7% |
Other | 11 | 15% |
Unknown | 12 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 21 | 30% |
Biochemistry, Genetics and Molecular Biology | 11 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 10 | 14% |
Neuroscience | 5 | 7% |
Chemistry | 3 | 4% |
Other | 5 | 7% |
Unknown | 16 | 23% |