Title |
Sequencing quality assessment tools to enable data-driven informatics for high throughput genomics
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Published in |
Frontiers in Genetics, January 2013
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DOI | 10.3389/fgene.2013.00288 |
Pubmed ID | |
Authors |
Richard M. Leggett, Ricardo H. Ramirez-Gonzalez, Bernardo J. Clavijo, Darren Waite, Robert P. Davey |
Abstract |
The processes of quality assessment and control are an active area of research at The Genome Analysis Centre (TGAC). Unlike other sequencing centers that often concentrate on a certain species or technology, TGAC applies expertise in genomics and bioinformatics to a wide range of projects, often requiring bespoke wet lab and in silico workflows. TGAC is fortunate to have access to a diverse range of sequencing and analysis platforms, and we are at the forefront of investigations into library quality and sequence data assessment. We have developed and implemented a number of algorithms, tools, pipelines and packages to ascertain, store, and expose quality metrics across a number of next-generation sequencing platforms, allowing rapid and in-depth cross-platform Quality Control (QC) bioinformatics. In this review, we describe these tools as a vehicle for data-driven informatics, offering the potential to provide richer context for downstream analysis and to inform experimental design. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 12 | 27% |
United States | 9 | 20% |
France | 3 | 7% |
Switzerland | 3 | 7% |
Netherlands | 2 | 4% |
Canada | 2 | 4% |
Germany | 1 | 2% |
Norway | 1 | 2% |
India | 1 | 2% |
Other | 2 | 4% |
Unknown | 9 | 20% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 28 | 62% |
Members of the public | 16 | 36% |
Science communicators (journalists, bloggers, editors) | 1 | 2% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 7 | 2% |
Netherlands | 3 | <1% |
Italy | 3 | <1% |
United States | 3 | <1% |
Argentina | 2 | <1% |
Brazil | 2 | <1% |
France | 1 | <1% |
South Africa | 1 | <1% |
Canada | 1 | <1% |
Other | 5 | 1% |
Unknown | 385 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 82 | 20% |
Student > Master | 59 | 14% |
Student > Bachelor | 58 | 14% |
Student > Ph. D. Student | 55 | 13% |
Student > Postgraduate | 22 | 5% |
Other | 48 | 12% |
Unknown | 89 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 142 | 34% |
Biochemistry, Genetics and Molecular Biology | 100 | 24% |
Computer Science | 14 | 3% |
Medicine and Dentistry | 13 | 3% |
Immunology and Microbiology | 11 | 3% |
Other | 35 | 8% |
Unknown | 98 | 24% |