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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Methylated DNA is over-represented in whole-genome bisulfite sequencing data
|
|---|---|
| Published in |
Frontiers in Genetics, October 2014
|
| DOI | 10.3389/fgene.2014.00341 |
| Pubmed ID | |
| Authors |
Lexiang Ji, Takahiko Sasaki, Xiaoxiao Sun, Ping Ma, Zachary A. Lewis, Robert J. Schmitz |
| Abstract |
The development of whole-genome bisulfite sequencing (WGBS) has resulted in a number of exciting discoveries about the role of DNA methylation leading to a plethora of novel testable hypotheses. Methods for constructing sodium bisulfite-converted and amplified libraries have recently advanced to the point that the bottleneck for experiments that use WGBS has shifted to data analysis and interpretation. Here we present empirical evidence for an over-representation of reads from methylated DNA in WGBS. This enrichment for methylated DNA is exacerbated by higher cycles of PCR and is influenced by the type of uracil-insensitive DNA polymerase used for amplifying the sequencing library. Future efforts to computationally correct for this enrichment bias will be essential to increasing the accuracy of determining methylation levels for individual cytosines. It is especially critical for studies that seek to accurately quantify DNA methylation levels in populations that may segregate for allelic DNA methylation states. |
X Demographics
The data shown below were collected from the profiles of 20 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 5 | 25% |
| United States | 4 | 20% |
| Switzerland | 2 | 10% |
| Germany | 1 | 5% |
| Georgia | 1 | 5% |
| India | 1 | 5% |
| Unknown | 6 | 30% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 10 | 50% |
| Scientists | 9 | 45% |
| Science communicators (journalists, bloggers, editors) | 1 | 5% |
Mendeley readers
The data shown below were compiled from readership statistics for 166 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 2% |
| United Kingdom | 2 | 1% |
| Switzerland | 1 | <1% |
| Australia | 1 | <1% |
| Netherlands | 1 | <1% |
| Italy | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 156 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 47 | 28% |
| Student > Ph. D. Student | 40 | 24% |
| Student > Master | 20 | 12% |
| Student > Bachelor | 14 | 8% |
| Professor > Associate Professor | 8 | 5% |
| Other | 17 | 10% |
| Unknown | 20 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 73 | 44% |
| Biochemistry, Genetics and Molecular Biology | 44 | 27% |
| Computer Science | 7 | 4% |
| Chemistry | 4 | 2% |
| Mathematics | 3 | 2% |
| Other | 14 | 8% |
| Unknown | 21 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 May 2016.
All research outputs
#3,080,796
of 24,701,594 outputs
Outputs from Frontiers in Genetics
#803
of 13,311 outputs
Outputs of similar age
#34,432
of 265,328 outputs
Outputs of similar age from Frontiers in Genetics
#9
of 116 outputs
Altmetric has tracked 24,701,594 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,311 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,328 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 116 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.