Fecal egg counts for gastrointestinal nematodes are associated with a polymorphism in the MHC-DRB1 gene in the Iranian Ghezel sheep breed

Rahman Hajializadeh Valilou, Seyed A. Rafat, David R. Notter, Djalil Shojda, Gholamali Moghaddam, Ahmad Nematollahi

Genetic variation among sheep breeds in resistance to gastrointestinal nematodes (GIN) has been demonstrated in several production environments. Relationships between the ovine major histocompatibility complex and resistance to GIN have been studied, but few studies have systematically examined this issue in less-developed and semi-arid regions. The aim of the current study was to explore associations between fecal worm egg counts (FEC) for several GIN and polymorphisms in the DRB1 gene. One hundred male lambs were selected at 4-6 months of age from weaned animals in five flocks (n = 20 per flock). Body weights were determined, FAMACHA scores based on color of the ocular mucous membranes were assigned as an indicator of anemia, and blood and fecal samples were collected twice to evaluate FEC and blood packed cell volume (PCV) and for DNA isolation. A repeated-measures analysis of variance was used to test effects of genotype on FEC. The model included fixed effects of flock, genotype, time of measurement (1 or 2), and flock × time and genoype × time interactions, and a random (repeated) effect of lamb. Two genotypes (A1A1 and A1A2) were observed following digestion of Region 1 of Ovar-DRB1 with PstI. Genotypic frequencies were 0.73 for A1A1 and 0.27 for A1A2. FEC differed between Ovar_DRB1 genotypes A1A1 and A1A2 for Marshallagia marshalli, Strongyle, and total nematode FEC. Observed FEC were 30-41% lower for genotype A1A1. Differences among genotypes were consistent across measurement times, with no effect of genotype × measurement time interaction for any parasite class (P ≥ 0.34). A significant association was observed between FAMACHA scores and lamb PCV, and the residual correlation between these two variables was -0.51 (P < 0.001). FAMACHA scores can thus be used to detect differences among lambs in PCV, and polymorphic markers of Ovar-DRB1 have potential value as an indicator of parasite resistance in applied animal breeding programs on sheep farms in this region.