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Development of SSR Markers and Assessment of Genetic Diversity in Medicinal Chrysanthemum morifolium Cultivars

Overview of attention for article published in Frontiers in Genetics, June 2016
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Title
Development of SSR Markers and Assessment of Genetic Diversity in Medicinal Chrysanthemum morifolium Cultivars
Published in
Frontiers in Genetics, June 2016
DOI 10.3389/fgene.2016.00113
Pubmed ID
Authors

Shangguo Feng, Renfeng He, Jiangjie Lu, Mengying Jiang, Xiaoxia Shen, Yan Jiang, Zhi'an Wang, Huizhong Wang

Abstract

Chrysanthemum morifolium, is a well-known flowering plant worldwide, and has a high commercial, floricultural, and medicinal value. In this study, simple-sequence repeat (SSR) markers were generated from EST datasets and were applied to assess the genetic diversity among 32 cultivars. A total of 218 in silico SSR loci were identified from 7300 C. morifolium ESTs retrieved from GenBank. Of all SSR loci, 61.47% of them (134) were hexa-nucleotide repeats, followed by tri-nucleotide repeats (17.89%), di-nucleotide repeats (12.39%), tetra-nucleotide repeats (4.13%), and penta-nucleotide repeats (4.13%). In this study, 17 novel EST-SSR markers were verified. Along with 38 SSR markers reported previously, 55 C. morifolium SSR markers were selected for further genetic diversity analysis. PCR amplification of these EST-SSRs produced 1319 fragments, 1306 of which showed polymorphism. The average polymorphism information content of the SSR primer pairs was 0.972 (0.938-0.993), which showed high genetic diversity among C. morifolium cultivars. Based on SSR markers, 32 C. morifolium cultivars were separated into two main groups by partitioning of the clusters using the unweighted pair group method with arithmetic mean dendrogram, which was further supported by a principal coordinate analysis plot. Phylogenetic relationship among C. morifolium cultivars as revealed by SSR markers was highly consistent with the classification of medicinal C. morifolium populations according to their origin and ecological distribution. Our results demonstrated that SSR markers were highly reproducible and informative, and could be used to evaluate genetic diversity and relationships among medicinal C. morifolium cultivars.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 91 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 17 19%
Student > Ph. D. Student 13 14%
Student > Bachelor 8 9%
Student > Master 7 8%
Student > Postgraduate 4 4%
Other 11 12%
Unknown 31 34%
Readers by discipline Count As %
Agricultural and Biological Sciences 40 44%
Biochemistry, Genetics and Molecular Biology 14 15%
Unspecified 1 1%
Environmental Science 1 1%
Pharmacology, Toxicology and Pharmaceutical Science 1 1%
Other 2 2%
Unknown 32 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 June 2016.
All research outputs
#18,463,662
of 22,877,793 outputs
Outputs from Frontiers in Genetics
#7,070
of 11,919 outputs
Outputs of similar age
#266,958
of 352,336 outputs
Outputs of similar age from Frontiers in Genetics
#51
of 61 outputs
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