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X Demographics
Mendeley readers
Attention Score in Context
| Title |
T Lymphocyte Myosin IIA is Required for Maturation of the Immunological Synapse
|
|---|---|
| Published in |
Frontiers in immunology, January 2012
|
| DOI | 10.3389/fimmu.2012.00230 |
| Pubmed ID | |
| Authors |
Sudha Kumari, Santosha Vardhana, Michael Cammer, Silvia Curado, Luis Santos, Michael P. Sheetz, Michael L. Dustin |
| Abstract |
The role of non-muscle myosin IIA (heavy chain encoded by the non-muscle myosin heavy chain 9 gene, Myh9) in immunological synapse formation is controversial. We have addressed the role of myosin IIA heavy chain protein (MYH9) in mouse T cells responding to MHC-peptide complexes and ICAM-1 in supported planar bilayers - a model for immunological synapse maturation. We found that reduction of MYH9 expression levels using Myh9 siRNA in proliferating mouse CD4(+) AND T cell receptor (TCR) transgenic T cells resulted in increased spreading area, failure to assemble the central and peripheral supramolecular activation clusters (cSMAC and pSMAC), and increased motility. Surprisingly, TCR microcluster speed was reduced marginally, however TCR microclusters dissipated prior to forming a cSMAC. TCR microclusters formed in the Myh9 siRNA-treated T cells showed reduced phosphorylation of the Src family kinase (SFK) activation loop and displayed reduced cytoplasmic calcium ion (Ca(2+)) elevation. In addition, Myh9 siRNA-treated cells displayed reduced phosphorylation of the Cas-L substrate domain - a force-dependent SFK substrate - which was observed in control siRNA-treated cells in foci throughout the immunological synapse except the cSMAC. Cas-L exhibited TCR ligation-dependent induction of phosphorylation. These results provide further evidence that T cell activation is modulated by intrinsic force-generating systems and can be viewed as a mechanically responsive process influenced by MYH9. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 91 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 2% |
| Japan | 1 | 1% |
| Portugal | 1 | 1% |
| France | 1 | 1% |
| Unknown | 86 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 28 | 31% |
| Researcher | 19 | 21% |
| Student > Master | 13 | 14% |
| Student > Postgraduate | 4 | 4% |
| Professor > Associate Professor | 4 | 4% |
| Other | 11 | 12% |
| Unknown | 12 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 36 | 40% |
| Immunology and Microbiology | 14 | 15% |
| Biochemistry, Genetics and Molecular Biology | 12 | 13% |
| Physics and Astronomy | 3 | 3% |
| Economics, Econometrics and Finance | 2 | 2% |
| Other | 11 | 12% |
| Unknown | 13 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 March 2016.
All research outputs
#16,881,298
of 25,604,262 outputs
Outputs from Frontiers in immunology
#18,585
of 32,042 outputs
Outputs of similar age
#169,800
of 251,039 outputs
Outputs of similar age from Frontiers in immunology
#111
of 275 outputs
Altmetric has tracked 25,604,262 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 32,042 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.4. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 251,039 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 275 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.