You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
Preparing Unbiased T-Cell Receptor and Antibody cDNA Libraries for the Deep Next Generation Sequencing Profiling
|
|---|---|
| Published in |
Frontiers in immunology, January 2013
|
| DOI | 10.3389/fimmu.2013.00456 |
| Pubmed ID | |
| Authors |
Ilgar Z. Mamedov, Olga V. Britanova, Ivan V. Zvyagin, Maria A. Turchaninova, Dmitriy A. Bolotin, Ekaterina V. Putintseva, Yuriy B. Lebedev, Dmitriy M. Chudakov |
| Abstract |
High-throughput sequencing has the power to reveal the nature of adaptive immunity as represented by the full complexity of T-cell receptor (TCR) and antibody (IG) repertoires, but is at present severely compromised by the quantitative bias, bottlenecks, and accumulated errors that inevitably occur in the course of library preparation and sequencing. Here we report an optimized protocol for the unbiased preparation of TCR and IG cDNA libraries for high-throughput sequencing, starting from thousands or millions of live cells in an investigated sample. Critical points to control are revealed, along with tips that allow researchers to minimize quantitative bias, accumulated errors, and cross-sample contamination at each stage, and to enhance the subsequent bioinformatic analysis. The protocol is simple, reliable, and can be performed in 1-2 days. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Czechia | 1 | 33% |
| United States | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 266 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 3 | 1% |
| United States | 2 | <1% |
| Japan | 1 | <1% |
| Unknown | 260 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 62 | 23% |
| Student > Ph. D. Student | 58 | 22% |
| Student > Master | 27 | 10% |
| Student > Bachelor | 20 | 8% |
| Student > Doctoral Student | 19 | 7% |
| Other | 44 | 17% |
| Unknown | 36 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 79 | 30% |
| Biochemistry, Genetics and Molecular Biology | 57 | 21% |
| Immunology and Microbiology | 42 | 16% |
| Medicine and Dentistry | 25 | 9% |
| Engineering | 4 | 2% |
| Other | 20 | 8% |
| Unknown | 39 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2021.
All research outputs
#2,376,843
of 25,374,647 outputs
Outputs from Frontiers in immunology
#2,307
of 31,516 outputs
Outputs of similar age
#22,452
of 288,991 outputs
Outputs of similar age from Frontiers in immunology
#23
of 503 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 31,516 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.4. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 288,991 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 503 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.