Title |
Hepatitis C-Associated Mixed Cryoglobulinemic Vasculitis Induces Differential Gene Expression in Peripheral Mononuclear Cells
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Published in |
Frontiers in immunology, May 2014
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DOI | 10.3389/fimmu.2014.00248 |
Pubmed ID | |
Authors |
Sreetha Sidharthan, Cheol-Woo Kim, Alison A. Murphy, Xiaozhen Zhang, Jun Yang, Richard A. Lempicki, Michael C. Sneller, Shyam Kottilil |
Abstract |
This study examines the distinct gene expression profile of peripheral blood mononuclear cells from patients with chronic hepatitis C infection and mixed cryoglobulinemic (MC) vasculitis. Our DNA microarray analysis indicates that hepatitis C virus (HCV)-associated MC vasculitis is characterized by compromised neutrophil function, impaired chemotaxis, and increased interferon-stimulated gene (ISG) expression, contributing to overall MC pathogenesis and end-organ damage. Increased ISG expression is suggestive of an enhanced endogenous interferon gene signature. PBMC depletion assays demonstrate that this increased expression is likely due to an activation of monocytes and not a direct result of B cell expansion. Notably, this monocyte activation of ISG expression in HCV-associated MC vasculitis suggests a poor predictor status of interferon-based treatment. Further analysis of PBMC gene expression profiles before and after in vivo B cell depletion therapy is critical to completely understanding the mechanisms of MC vasculitis pathogenesis. |
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Switzerland | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 4 | 36% |
Student > Master | 2 | 18% |
Student > Bachelor | 1 | 9% |
Student > Doctoral Student | 1 | 9% |
Student > Postgraduate | 1 | 9% |
Other | 0 | 0% |
Unknown | 2 | 18% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 18% |
Agricultural and Biological Sciences | 2 | 18% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
Environmental Science | 1 | 9% |
Other | 0 | 0% |
Unknown | 2 | 18% |