Title |
A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex–Peptide Monomers by Flow Cytometry
|
---|---|
Published in |
Frontiers in immunology, February 2017
|
DOI | 10.3389/fimmu.2017.00096 |
Pubmed ID | |
Authors |
P. Anoop Chandran, Sonja Heidu, Henning Zelba, Barbara Schmid-Horch, Hans-Georg Rammensee, Steve Pascolo, Cécile Gouttefangeas |
Abstract |
Major histocompatibility complex (MHC) multimers are essential tools in T cell immunomonitoring, which are employed both in basic and clinical research, as well as for assessing clinical samples during therapy. The generation of MHC monomers loaded with synthetic peptides is an elaborate and time-consuming process. It would be beneficial to assess the quality of these monomers prior to downstream applications. In this technical note, we describe a novel flow cytometry-based, cell-free, quick, and robust assay to check the quality of MHC monomers directly after refolding or after long-term storage. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 14% |
Switzerland | 1 | 14% |
Unknown | 5 | 71% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 7 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 30% |
Student > Bachelor | 5 | 19% |
Student > Ph. D. Student | 3 | 11% |
Student > Postgraduate | 2 | 7% |
Student > Master | 1 | 4% |
Other | 3 | 11% |
Unknown | 5 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 26% |
Immunology and Microbiology | 5 | 19% |
Biochemistry, Genetics and Molecular Biology | 4 | 15% |
Engineering | 2 | 7% |
Computer Science | 1 | 4% |
Other | 3 | 11% |
Unknown | 5 | 19% |