Title |
Pathogen-Reactive T Helper Cell Analysis in the Pig
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Published in |
Frontiers in immunology, May 2017
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DOI | 10.3389/fimmu.2017.00565 |
Pubmed ID | |
Authors |
Friederike Ebner, Patrycja Schwiertz, Svenja Steinfelder, Robert Pieper, Jürgen Zentek, Nicole Schütze, Christoph G. Baums, Gottfried Alber, Peter Geldhof, Susanne Hartmann |
Abstract |
There is growing interest in studying host-pathogen interactions in human-relevant large animal models such as the pig. Despite the progress in developing immunological reagents for porcine T cell research, there is an urgent need to directly assess pathogen-specific T cells-an extremely rare population of cells, but of upmost importance in orchestrating the host immune response to a given pathogen. Here, we established that the activation marker CD154 (CD40L), known from human and mouse studies, identifies also porcine antigen-reactive CD4(+) T lymphocytes. CD154 expression was upregulated early after antigen encounter and CD4(+)CD154(+) antigen-reactive T cells coexpressed cytokines. Antigen-induced expansion and autologous restimulation enabled a time- and dose-resolved analysis of CD154 regulation and a significantly increased resolution in phenotypic profiling of antigen-responsive cells. CD154 expression identified T cells responding to staphylococcal Enterotoxin B superantigen stimulation as well as T cells responding to the fungus Candida albicans and T cells specific for a highly prevalent intestinal parasite, the nematode Ascaris suum during acute and trickle infection. Antigen-reactive T cells were further detected after immunization of pigs with a single recombinant bacterial antigen of Streptococcus suis only. Thus, our study offers new ways to study antigen-specific T lymphocytes in the pig and their contribution to host-pathogen interactions. |
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Country | Count | As % |
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Belgium | 1 | 25% |
United States | 1 | 25% |
Switzerland | 1 | 25% |
United Kingdom | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 75% |
Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 35 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 8 | 23% |
Student > Ph. D. Student | 5 | 14% |
Student > Master | 5 | 14% |
Student > Bachelor | 2 | 6% |
Student > Doctoral Student | 1 | 3% |
Other | 3 | 9% |
Unknown | 11 | 31% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 5 | 14% |
Immunology and Microbiology | 5 | 14% |
Agricultural and Biological Sciences | 3 | 9% |
Nursing and Health Professions | 1 | 3% |
Other | 3 | 9% |
Unknown | 11 | 31% |