Title |
Endoplasmic Reticulum Stress Sensor IRE1α Enhances IL-23 Expression by Human Dendritic Cells
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Published in |
Frontiers in immunology, June 2017
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DOI | 10.3389/fimmu.2017.00639 |
Pubmed ID | |
Authors |
Saioa Márquez, José Javier Fernández, Eli Terán-Cabanillas, Carmen Herrero, Sara Alonso, Alicia Azogil, Olimpio Montero, Takao Iwawaki, Juan R. Cubillos-Ruiz, Nieves Fernández, Mariano Sánchez Crespo |
Abstract |
Human monocyte-derived dendritic cells (DCs) exposed to pathogen-associated molecular patterns (PAMPs) undergo bioenergetic changes that influence the immune response. We found that stimulation with PAMPs enhanced glycolysis in DCs, whereas oxidative phosphorylation remained unaltered. Glucose starvation and the hexokinase inhibitor 2-deoxy-d-glucose (2-DG) modulated cytokine expression in stimulated DCs. Strikingly, IL23A was markedly induced upon 2-DG treatment, but not during glucose deprivation. Since 2-DG can also rapidly inhibit protein N-glycosylation, we postulated that this compound could induce IL-23 in DCs via activation of the endoplasmic reticulum (ER) stress response. Indeed, stimulation of DCs with PAMPs in the presence of 2-DG robustly activated inositol-requiring protein 1α (IRE1α) signaling and to a lesser extent the PERK arm of the unfolded protein response. Additional ER stressors such as tunicamycin and thapsigargin also promoted IL-23 expression by PAMP-stimulated DCs. Pharmacological, biochemical, and genetic analyses using conditional knockout mice revealed that IL-23 induction in ER stressed DCs stimulated with PAMPs was IRE1α/X-box binding protein 1-dependent upon zymosan stimulation. Interestingly, we further evidenced PERK-mediated and CAAT/enhancer-binding protein β-dependent trans-activation of IL23A upon lipopolysaccharide treatment. Our findings uncover that the ER stress response can potently modulate cytokine expression in PAMP-stimulated human DCs. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Switzerland | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 67 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 15 | 22% |
Researcher | 12 | 18% |
Student > Doctoral Student | 10 | 15% |
Student > Bachelor | 9 | 13% |
Student > Postgraduate | 3 | 4% |
Other | 6 | 9% |
Unknown | 12 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 18 | 27% |
Biochemistry, Genetics and Molecular Biology | 15 | 22% |
Agricultural and Biological Sciences | 9 | 13% |
Medicine and Dentistry | 6 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
Other | 5 | 7% |
Unknown | 12 | 18% |