Title |
CRISPRi Induced Suppression of Fimbriae Gene (fimH) of a Uropathogenic Escherichia coli: An Approach to Inhibit Microbial Biofilms
|
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Published in |
Frontiers in immunology, November 2017
|
DOI | 10.3389/fimmu.2017.01552 |
Pubmed ID | |
Authors |
Azna Zuberi, Nayeem Ahmad, Asad U. Khan |
Abstract |
Urinary tract infection (UTI) is one the common infections caused by the recalcitrant nature of biofilms, developed after the pathogen has adhered to the inner lining of the urinary tract. Although significant research has been made in recent years to control these types of infection, but as of yet, no approach has sufficiently been able to reduce the prevalence of UTIs. The main objective of this study was to prevent UTIs through targeting the fimH gene, which is the major virulent factor responsible for biofilm formation. The novelty of this work lies in the use of CRISPRi, a gene specific editing tool to control such types of infections. Accordingly, the system was designed to target fimH gene, responsible for bacterial adherence and this approach was successfully validated by performing microscopic, biofilm and adherence assays. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 33% |
Singapore | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Scientists | 2 | 67% |
Members of the public | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 51 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 13 | 25% |
Student > Bachelor | 5 | 10% |
Researcher | 5 | 10% |
Professor | 2 | 4% |
Professor > Associate Professor | 2 | 4% |
Other | 5 | 10% |
Unknown | 19 | 37% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 14% |
Immunology and Microbiology | 5 | 10% |
Biochemistry, Genetics and Molecular Biology | 5 | 10% |
Nursing and Health Professions | 3 | 6% |
Engineering | 2 | 4% |
Other | 5 | 10% |
Unknown | 24 | 47% |