Title |
NETQUANT: Automated Quantification of Neutrophil Extracellular Traps
|
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Published in |
Frontiers in immunology, January 2018
|
DOI | 10.3389/fimmu.2017.01999 |
Pubmed ID | |
Authors |
Tirthankar Mohanty, Ole E Sørensen, Pontus Nordenfelt |
Abstract |
Neutrophil extracellular traps (NETs) that are extensive webs of DNA covered with antimicrobial proteins into the extracellular environment during infection or inflammation as a part of their defense arsenal. Image acquisition of fluorescently labeled NETs and subsequent image-based quantification is frequently used to analyze NET formation (NETosis) in response to various stimuli. However, there are important limitations in the present methods for quantification. Manual methods tend to be error-prone, tedious, and often quite subjective, whereas the software-rooted options are either semi-automatic or difficult to operate. Here, we present an automated and uncomplicated approach for quantifying NETs from fluorescence images, built as a freely available app for MATLAB®. It is based on detection of a set of clearly defined parameters, all related to the biological manifestation of NETs and allowing for single-cell resolution quantification and analysis. |
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Geographical breakdown
Country | Count | As % |
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Sweden | 2 | 40% |
Switzerland | 1 | 20% |
Unknown | 2 | 40% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 80% |
Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 44 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 8 | 18% |
Student > Master | 6 | 14% |
Student > Bachelor | 4 | 9% |
Student > Ph. D. Student | 4 | 9% |
Student > Doctoral Student | 2 | 5% |
Other | 6 | 14% |
Unknown | 14 | 32% |
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Agricultural and Biological Sciences | 7 | 16% |
Immunology and Microbiology | 5 | 11% |
Medicine and Dentistry | 4 | 9% |
Engineering | 2 | 5% |
Other | 3 | 7% |
Unknown | 14 | 32% |