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Mendeley readers
| Title |
Variable Domain N-Linked Glycans Acquired During Antigen-Specific Immune Responses Can Contribute to Immunoglobulin G Antibody Stability
|
|---|---|
| Published in |
Frontiers in immunology, April 2018
|
| DOI | 10.3389/fimmu.2018.00740 |
| Pubmed ID | |
| Authors |
Fleur S. van de Bovenkamp, Ninotska I. L. Derksen, Mariëlle J. van Breemen, Steven W. de Taeye, Pleuni Ooijevaar-de Heer, Rogier W. Sanders, Theo Rispens |
| Abstract |
Immunoglobulin G (IgG) can contain N-linked glycans in the variable domains, the so-called Fab glycans, in addition to the Fc glycans in the CH2 domains. These Fab glycans are acquired following introduction of N-glycosylation sites during somatic hypermutation and contribute to antibody diversification. We investigated whether Fab glycans may-in addition to affecting antigen binding-contribute to antibody stability. By analyzing thermal unfolding profiles of antibodies with or without Fab glycans, we demonstrate that introduction of Fab glycans can improve antibody stability. Strikingly, removal of Fab glycans naturally acquired during antigen-specific immune responses can deteriorate antibody stability, suggesting in vivo selection of stable, glycosylated antibodies. Collectively, our data show that variable domain N-linked glycans acquired during somatic hypermutation can contribute to IgG antibody stability. These findings indicate that introducing Fab glycans may represent a mechanism to improve therapeutic/diagnostic antibody stability. |
Mendeley readers
The data shown below were compiled from readership statistics for 68 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 68 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 22% |
| Student > Ph. D. Student | 15 | 22% |
| Student > Bachelor | 6 | 9% |
| Professor | 4 | 6% |
| Student > Master | 4 | 6% |
| Other | 5 | 7% |
| Unknown | 19 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 22 | 32% |
| Immunology and Microbiology | 9 | 13% |
| Agricultural and Biological Sciences | 6 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 6% |
| Chemical Engineering | 3 | 4% |
| Other | 5 | 7% |
| Unknown | 19 | 28% |