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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Novel Engraftment and T Cell Differentiation of Human Hematopoietic Cells in ART−/− IL2RG−/Y SCID Pigs
|
|---|---|
| Published in |
Frontiers in immunology, February 2020
|
| DOI | 10.3389/fimmu.2020.00100 |
| Pubmed ID | |
| Authors |
Adeline N. Boettcher, Yunsheng Li, Amanda P. Ahrens, Matti Kiupel, Kristen A. Byrne, Crystal L. Loving, A. Giselle Cino-Ozuna, Jayne E. Wiarda, Malavika Adur, Blythe Schultz, Jack J. Swanson, Elizabeth M. Snella, Chak-Sum Ho, Sara E. Charley, Zoe E. Kiefer, Joan E. Cunnick, Ellie J. Putz, Giuseppe Dell'Anna, Jackie Jens, Swanand Sathe, Frederick Goldman, Erik R. Westin, Jack C. M. Dekkers, Jason W. Ross, Christopher K. Tuggle |
| Abstract |
Pigs with severe combined immunodeficiency (SCID) are an emerging biomedical animal model. Swine are anatomically and physiologically more similar to humans than mice, making them an invaluable tool for preclinical regenerative medicine and cancer research. One essential step in further developing this model is the immunological humanization of SCID pigs. In this work we have generated T- B- NK- SCID pigs through site directed CRISPR/Cas9 mutagenesis of IL2RG within a naturally occurring DCLRE1C (ARTEMIS)-/- genetic background. We confirmed ART-/-IL2RG-/Y pigs lacked T, B, and NK cells in both peripheral blood and lymphoid tissues. Additionally, we successfully performed a bone marrow transplant on one ART-/-IL2RG-/Y male SCID pig with bone marrow from a complete swine leukocyte antigen (SLA) matched donor without conditioning to reconstitute porcine T and NK cells. Next, we performed in utero injections of cultured human CD34+ selected cord blood cells into the fetal ART-/-IL2RG-/Y SCID pigs. At birth, human CD45+ CD3ε+ cells were detected in cord and peripheral blood of in utero injected SCID piglets. Human leukocytes were also detected within the bone marrow, spleen, liver, thymus, and mesenteric lymph nodes of these animals. Taken together, we describe critical steps forwards the development of an immunologically humanized SCID pig model. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Russia | 1 | 25% |
| Switzerland | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 38 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 16% |
| Other | 4 | 11% |
| Student > Bachelor | 3 | 8% |
| Student > Ph. D. Student | 3 | 8% |
| Student > Master | 3 | 8% |
| Other | 6 | 16% |
| Unknown | 13 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 18% |
| Biochemistry, Genetics and Molecular Biology | 6 | 16% |
| Medicine and Dentistry | 5 | 13% |
| Immunology and Microbiology | 3 | 8% |
| Business, Management and Accounting | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 13 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 162. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 June 2021.
All research outputs
#253,978
of 25,611,630 outputs
Outputs from Frontiers in immunology
#259
of 32,048 outputs
Outputs of similar age
#6,707
of 474,627 outputs
Outputs of similar age from Frontiers in immunology
#5
of 584 outputs
Altmetric has tracked 25,611,630 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 32,048 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.4. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 474,627 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 584 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.