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X Demographics
Mendeley readers
Attention Score in Context
| Title |
GMP-Compliant Universal Antigen Presenting Cells (uAPC) Promote the Metabolic Fitness and Antitumor Activity of Armored Cord Blood CAR-NK Cells
|
|---|---|
| Published in |
Frontiers in immunology, February 2021
|
| DOI | 10.3389/fimmu.2021.626098 |
| Pubmed ID | |
| Authors |
Enli Liu, Sonny O. T. Ang, Lucila Kerbauy, Rafet Basar, Indreshpal Kaur, Mecit Kaplan, Li Li, Yijiu Tong, May Daher, Emily L. Ensley, Nadima Uprety, Ana Karen Nunez Cortes, Ryan Z. Yang, Ye Li, Hila Shaim, Francia Reyes Silva, Paul Lin, Vakul Mohanty, Sunil Acharya, Mayra Shanley, Luis Muniz-Feliciano, Pinaki P. Banerjee, Ken Chen, Richard E. Champlin, Elizabeth J. Shpall, Katayoun Rezvani |
| Abstract |
Natural killer (NK) cells are innate lymphocytes recognized for their important role against tumor cells. NK cells expressing chimeric antigen receptors (CARs) have enhanced effector function against various type of cancer and are attractive contenders for the next generation of cancer immunotherapies. However, a number of factors have hindered the application of NK cells for cellular therapy, including their poor in vitro growth kinetics and relatively low starting percentages within the mononuclear cell fraction of peripheral blood or cord blood (CB). To overcome these limitations, we genetically-engineered human leukocyte antigen (HLA)-A- and HLA-B- K562 cells to enforce the expression of CD48, 4-1BBL, and membrane-bound IL-21 (mbIL21), creating a universal antigen presenting cell (uAPC) capable of stimulating their cognate receptors on NK cells. We have shown that uAPC can drive the expansion of both non-transduced (NT) and CAR-transduced CB derived NK cells by >900-fold in 2 weeks of co-culture with excellent purity (>99.9%) and without indications of senescence/exhaustion. We confirmed that uAPC-expanded research- and clinical-grade NT and CAR-transduced NK cells have higher metabolic fitness and display enhanced effector function against tumor targets compared to the corresponding cell fractions cultured without uAPCs. This novel approach allowed the expansion of highly pure GMP-grade CAR NK cells at optimal cell numbers to be used for adoptive CAR NK cell-based cancer immunotherapy. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 66 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 14 | 21% |
| Student > Ph. D. Student | 8 | 12% |
| Other | 6 | 9% |
| Student > Master | 3 | 5% |
| Student > Bachelor | 3 | 5% |
| Other | 3 | 5% |
| Unknown | 29 | 44% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 11 | 17% |
| Biochemistry, Genetics and Molecular Biology | 10 | 15% |
| Medicine and Dentistry | 5 | 8% |
| Agricultural and Biological Sciences | 4 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 3% |
| Other | 4 | 6% |
| Unknown | 30 | 45% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 December 2021.
All research outputs
#7,219,481
of 25,864,668 outputs
Outputs from Frontiers in immunology
#8,013
of 32,510 outputs
Outputs of similar age
#154,498
of 454,487 outputs
Outputs of similar age from Frontiers in immunology
#396
of 1,246 outputs
Altmetric has tracked 25,864,668 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 32,510 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.4. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 454,487 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 1,246 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.