Title |
Interleukin(IL)-36α and IL-36γ Induce Proinflammatory Mediators from Human Colonic Subepithelial Myofibroblasts
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Published in |
Frontiers in Medicine, September 2015
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DOI | 10.3389/fmed.2015.00069 |
Pubmed ID | |
Authors |
Toshihiro Kanda, Atsushi Nishida, Kenichiro Takahashi, Kentaro Hidaka, Hirotsugu Imaeda, Osamu Inatomi, Shigeki Bamba, Mitsushige Sugimoto, Akira Andoh |
Abstract |
Interleukin (IL)-36 cytokines are recently reported member of the IL-1 cytokine family. However, there is little information regarding the association between IL-36 cytokines and gut inflammation. In the present study, we investigated the biological activity of IL-36α and IL-36γ using human colonic subepithelial myofibroblasts (SEMFs). The mRNA expression and the protein expression of target molecules in SEMFs were evaluated using real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The intracellular signaling of IL-36 cytokines was analyzed using Western blot analysis and small interfering RNAs (siRNAs) specific for MyD88 adaptor proteins (MyD88 and IRAK1) and NF-κB p65. IL-36α and IL-36γ significantly enhanced the secretion of IL-6 and CXC chemokines (CXCL1, CXCL2, and CXCL8) by SEMFs. The combination of IL-36α/γ and IL-17A or of IL-36α/γ and tumor necrosis factor-α showed a synergistic effect on the induction of IL-6 and CXC chemokines. The mRNA expression of proinflammatory mediators induced by IL-36α and/or IL-36γ was significantly suppressed by transfection of siRNA for MyD88 or IRAK1. Both inhibitors of mitogen activated protein kinases and siRNAs specific for NF-κBp65 significantly reduced the expression of IL-6 and CXC chemokines induced by IL-36α and/or IL-36γ. These results suggest that IL-36α and IL-36γ contribute to gut inflammation through the induction of proinflammatory mediators. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 24 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 4 | 17% |
Student > Ph. D. Student | 4 | 17% |
Student > Master | 3 | 13% |
Student > Doctoral Student | 2 | 8% |
Researcher | 2 | 8% |
Other | 3 | 13% |
Unknown | 6 | 25% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 8 | 33% |
Agricultural and Biological Sciences | 2 | 8% |
Biochemistry, Genetics and Molecular Biology | 1 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Other | 3 | 13% |
Unknown | 8 | 33% |