Title |
Fate and Survival of Campylobacter coli in Swine Manure at Various Temperatures
|
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Published in |
Frontiers in Microbiology, January 2011
|
DOI | 10.3389/fmicb.2011.00262 |
Pubmed ID | |
Authors |
Xuan Thanh Bui, Anders Wolff, Mogen Madsen, Dang Duong Bang |
Abstract |
Campylobacter coli is the most common Campylobacter species found in pig (95%), but the ability of this bacterium to survive in swine manure as well as the potential for causing human illness are poorly understood. We present here laboratory-scale experiments to investigate the effect of temperature on the survival of C. coli in spiked swine manure samples at temperatures from 4 to 52°C. The survival of C. coli during storage for 30 days was studied by three different methods: bacterial culture (plate counting), DNA qPCR, and mRNA RT-qPCR. The results indicate that C. coli could survive in swine manure up to 24 days at 4°C. At higher temperatures, this bacterium survived only 7 days (15°C) or 6 days (22°C) of storage. The survival of C. coli was extremely short (few hours) in samples incubated at 42 and 52°C. The results from the RT-qPCR method were consistent with the data from the bacterial culture method, indicating that it detected only viable C. coli cells, thus eliminating false-positive resulting from DNA from dead C. coli cells. |
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Unknown | 1 | 20% |
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Scientists | 2 | 40% |
Practitioners (doctors, other healthcare professionals) | 1 | 20% |
Mendeley readers
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Student > Bachelor | 6 | 17% |
Researcher | 5 | 14% |
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Professor | 3 | 9% |
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Medicine and Dentistry | 2 | 6% |
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Unknown | 7 | 20% |