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Mendeley readers
Attention Score in Context
| Title |
Detection of autotrophic verrucomicrobial methanotrophs in a geothermal environment using stable isotope probing
|
|---|---|
| Published in |
Frontiers in Microbiology, January 2012
|
| DOI | 10.3389/fmicb.2012.00303 |
| Pubmed ID | |
| Authors |
Christine E. Sharp, Matthew B. Stott, Peter F. Dunfield |
| Abstract |
Genomic analysis of the methanotrophic verrucomicrobium "Methylacidiphilum infernorum" strain V4 has shown that most pathways conferring its methanotrophic lifestyle are similar to those found in proteobacterial methanotrophs. However, due to the large sequence divergence of its methane monooxygenase-encoding genes (pmo), "universal" pmoA polymerase chain reaction (PCR) primers do not target these bacteria. Unlike proteobacterial methanotrophs, "Methylacidiphilum" fixes carbon autotrophically, and uses methane only for energy generation. As a result, techniques used to detect methanotrophs in the environment such as (13)CH(4)-stable isotope probing (SIP) and pmoA-targeted PCR do not detect verrucomicrobial methanotrophs, and they may have been overlooked in previous environmental studies. We developed a modified SIP technique to identify active methanotrophic Verrucomicrobia in the environment by labeling with (13)CO(2) and (13)CH(4), individually and in combination. Testing the protocol in "M. infernorum" strain V4 resulted in assimilation of (13)CO(2) but not (13)CH(4), verifying its autotrophic lifestyle. To specifically detect methanotrophs (as opposed to other autotrophs) via (13)CO(2)-SIP, a quantitative PCR (qPCR) assay specific for verrucomicrobial-pmoA genes was developed and used in combination with SIP. Incubation of an acidic, high-temperature geothermal soil with (13)CH(4) + (12)CO(2) caused little shift in the density distribution of verrucomicrobial-pmoA genes relative to controls. However, labeling with (13)CO(2) in combination with (12)CH(4) or (13)CH(4) induced a strong shift in the distribution of verrucomicrobial-pmoA genes towards the heavy DNA fractions. The modified SIP technique demonstrated that the primary methanotrophs active in the soil were autotrophs and belonged to the Verrucomicrobia. This is the first demonstration of autotrophic, non-proteobacterial methanotrophy in situ, and provides a tool to detect verrucomicrobial methanotrophs in other ecosystems. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 124 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 2% |
| Germany | 1 | <1% |
| Belgium | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 119 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 30 | 24% |
| Student > Ph. D. Student | 27 | 22% |
| Other | 13 | 10% |
| Student > Master | 11 | 9% |
| Student > Bachelor | 9 | 7% |
| Other | 18 | 15% |
| Unknown | 16 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 47 | 38% |
| Biochemistry, Genetics and Molecular Biology | 18 | 15% |
| Environmental Science | 15 | 12% |
| Earth and Planetary Sciences | 7 | 6% |
| Medicine and Dentistry | 4 | 3% |
| Other | 12 | 10% |
| Unknown | 21 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 October 2022.
All research outputs
#6,513,494
of 23,506,079 outputs
Outputs from Frontiers in Microbiology
#6,462
of 25,938 outputs
Outputs of similar age
#57,701
of 247,458 outputs
Outputs of similar age from Frontiers in Microbiology
#64
of 318 outputs
Altmetric has tracked 23,506,079 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 25,938 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 247,458 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 318 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.