Title |
Overview of the genetic tools in the Archaea
|
---|---|
Published in |
Frontiers in Microbiology, January 2012
|
DOI | 10.3389/fmicb.2012.00337 |
Pubmed ID | |
Authors |
Haruyuki Atomi, Tadayuki Imanaka, Toshiaki Fukui |
Abstract |
This section provides an overview of the genetic systems developed in the Archaea. Genetic manipulation is possible in many members of the halophiles, methanogens, Sulfolobus, and Thermococcales. We describe the selection/counterselection principles utilized in each of these groups, which consist of antibiotics and their resistance markers, and auxotrophic host strains and complementary markers. The latter strategy utilizes techniques similar to those developed in yeast. However, Archaea are resistant to many of the antibiotics routinely used for selection in the Bacteria, and a number of strategies specific to the Archaea have been developed. In addition, examples utilizing the genetic systems developed for each group will be briefly described. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Switzerland | 1 | 50% |
United States | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 2% |
Canada | 1 | <1% |
United Kingdom | 1 | <1% |
Mexico | 1 | <1% |
Slovenia | 1 | <1% |
Unknown | 163 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 38 | 22% |
Researcher | 23 | 13% |
Student > Bachelor | 22 | 13% |
Student > Master | 18 | 11% |
Professor > Associate Professor | 8 | 5% |
Other | 29 | 17% |
Unknown | 33 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 64 | 37% |
Biochemistry, Genetics and Molecular Biology | 42 | 25% |
Immunology and Microbiology | 8 | 5% |
Engineering | 5 | 3% |
Chemistry | 4 | 2% |
Other | 15 | 9% |
Unknown | 33 | 19% |