Title |
Global Molecular Analyses of Methane Metabolism in Methanotrophic Alphaproteobacterium, Methylosinus trichosporium OB3b. Part II. Metabolomics and 13C-Labeling Study
|
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Published in |
Frontiers in Microbiology, January 2013
|
DOI | 10.3389/fmicb.2013.00070 |
Pubmed ID | |
Authors |
Song Yang, Janet B. Matsen, Michael Konopka, Abigail Green-Saxena, Justin Clubb, Martin Sadilek, Victoria J. Orphan, David Beck, Marina G. Kalyuzhnaya |
Abstract |
In this work we use metabolomics and (13)C-labeling data to refine central metabolic pathways for methane utilization in Methylosinus trichosporium OB3b, a model alphaproteobacterial methanotrophic bacterium. We demonstrate here that similar to non-methane utilizing methylotrophic alphaproteobacteria the core metabolism of the microbe is represented by several tightly connected metabolic cycles, such as the serine pathway, the ethylmalonyl-CoA (EMC) pathway, and the citric acid (TCA) cycle. Both in silico estimations and stable isotope labeling experiments combined with single cell (NanoSIMS) and bulk biomass analyses indicate that a significantly larger portion of the cell carbon (over 60%) is derived from CO2 in this methanotroph. Our(13) C-labeling studies revealed an unusual topology of the assimilatory network in which phosph(enol) pyruvate/pyruvate interconversions are key metabolic switches. A set of additional pathways for carbon fixation are identified and discussed. |
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Switzerland | 1 | 100% |
Demographic breakdown
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Scientists | 1 | 100% |
Mendeley readers
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Demographic breakdown
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Researcher | 37 | 27% |
Student > Ph. D. Student | 30 | 22% |
Other | 13 | 10% |
Student > Master | 10 | 7% |
Student > Bachelor | 6 | 4% |
Other | 11 | 8% |
Unknown | 28 | 21% |
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Chemistry | 4 | 3% |
Other | 11 | 8% |
Unknown | 31 | 23% |