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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Bacteriophage T7 DNA polymerase – sequenase
|
|---|---|
| Published in |
Frontiers in Microbiology, April 2014
|
| DOI | 10.3389/fmicb.2014.00181 |
| Pubmed ID | |
| Authors |
Bin Zhu |
| Abstract |
An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: (1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; (2) high processivity; (3) high fidelity in the absence of proofreading/exonuclease activity; and (4) production of clear and uniform signals for detection. The DNA polymerase encoded by bacteriophage T7 is naturally endowed with or can be engineered to have all these characteristics. The chemically or genetically modified enzyme (Sequenase) expedited significantly the development of DNA sequencing technology. This article reviews the history of studies on T7 DNA polymerase with emphasis on the serial key steps leading to its use in DNA sequencing. Lessons from the study and development of T7 DNA polymerase have and will continue to enlighten the characterization of novel DNA polymerases from newly discovered microbes and their modification for use in biotechnology. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Greece | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 64 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 14 | 21% |
| Researcher | 13 | 20% |
| Student > Ph. D. Student | 7 | 11% |
| Student > Master | 7 | 11% |
| Student > Postgraduate | 6 | 9% |
| Other | 9 | 14% |
| Unknown | 10 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 24 | 36% |
| Agricultural and Biological Sciences | 14 | 21% |
| Immunology and Microbiology | 4 | 6% |
| Chemistry | 4 | 6% |
| Medicine and Dentistry | 3 | 5% |
| Other | 6 | 9% |
| Unknown | 11 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 June 2022.
All research outputs
#3,058,017
of 22,753,345 outputs
Outputs from Frontiers in Microbiology
#2,773
of 24,627 outputs
Outputs of similar age
#28,996
of 203,744 outputs
Outputs of similar age from Frontiers in Microbiology
#17
of 171 outputs
Altmetric has tracked 22,753,345 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 24,627 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 203,744 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 171 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.