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Assessing impacts of unconventional natural gas extraction on microbial communities in headwater stream ecosystems in Northwestern Pennsylvania

Overview of attention for article published in Frontiers in Microbiology, November 2014
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Title
Assessing impacts of unconventional natural gas extraction on microbial communities in headwater stream ecosystems in Northwestern Pennsylvania
Published in
Frontiers in Microbiology, November 2014
DOI 10.3389/fmicb.2014.00522
Pubmed ID
Authors

Ryan Trexler, Caroline Solomon, Colin J. Brislawn, Justin R. Wright, Abigail Rosenberger, Erin E. McClure, Alyssa M. Grube, Mark P. Peterson, Mehdi Keddache, Olivia U. Mason, Terry C. Hazen, Christopher J. Grant, Regina Lamendella

Abstract

Hydraulic fracturing and horizontal drilling have increased dramatically in Pennsylvania Marcellus shale formations, however the potential for major environmental impacts are still incompletely understood. High-throughput sequencing of the 16S rRNA gene was performed to characterize the microbial community structure of water, sediment, bryophyte, and biofilm samples from 26 headwater stream sites in northwestern Pennsylvania with different histories of fracking activity within Marcellus shale formations. Further, we describe the relationship between microbial community structure and environmental parameters measured. Approximately 3.2 million 16S rRNA gene sequences were retrieved from a total of 58 samples. Microbial community analyses showed significant reductions in species richness as well as evenness in sites with Marcellus shale activity. Beta diversity analyses revealed distinct microbial community structure between sites with and without Marcellus shale activity. For example, operational taxonomic units (OTUs) within the Acetobacteracea, Methylocystaceae, Acidobacteriaceae, and Phenylobacterium were greater than three log-fold more abundant in MSA+ sites as compared to MSA- sites. Further, several of these OTUs were strongly negatively correlated with pH and positively correlated with the number of wellpads in a watershed. It should be noted that many of the OTUs enriched in MSA+ sites are putative acidophilic and/or methanotrophic populations. This study revealed apparent shifts in the autochthonous microbial communities and highlighted potential members that could be responding to changing stream conditions as a result of nascent industrial activity in these aquatic ecosystems.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 79 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 3%
Canada 1 1%
Unknown 76 96%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 18 23%
Researcher 15 19%
Student > Doctoral Student 11 14%
Student > Master 8 10%
Student > Ph. D. Student 8 10%
Other 15 19%
Unknown 4 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 30 38%
Environmental Science 14 18%
Biochemistry, Genetics and Molecular Biology 6 8%
Earth and Planetary Sciences 6 8%
Social Sciences 3 4%
Other 13 16%
Unknown 7 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2014.
All research outputs
#14,138,089
of 22,764,165 outputs
Outputs from Frontiers in Microbiology
#12,176
of 24,656 outputs
Outputs of similar age
#135,228
of 262,198 outputs
Outputs of similar age from Frontiers in Microbiology
#116
of 197 outputs
Altmetric has tracked 22,764,165 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,656 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,198 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 197 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.