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New metabolic pathway for degradation of 2-nitrobenzoate by Arthrobacter sp. SPG

Overview of attention for article published in Frontiers in Microbiology, June 2015
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Title
New metabolic pathway for degradation of 2-nitrobenzoate by Arthrobacter sp. SPG
Published in
Frontiers in Microbiology, June 2015
DOI 10.3389/fmicb.2015.00551
Pubmed ID
Authors

Pankaj K. Arora, Ashutosh Sharma

Abstract

Arthrobacter sp. SPG utilized 2-nitrobenzoate as its sole source of carbon and energy and degraded it with accumulation of stoichiometric amounts of nitrite ions. Salicylate and catechol were detected as metabolites of the 2-nitrobenzoate degradation using high performance liquid chromatography and gas chromatography-mass spectrometry. Enzyme activities for 2-nitrobenzoate-2-monooxygenase, salicylate hydroxylase, and catechol-1,2-dioxygenase were detected in the crude extracts of the 2-nitrobenzoate-induced cells of strain SPG. The 2-nitrobenzoate-monooxygenase activity resulted in formation of salicylate and nitrite from 2-nitrobenzoate, whereas salicylate hydroxylase catalyzed the conversion of salicylate to catechol. The ring-cleaving enzyme, catechol-1,2-dioxygenase cleaved catechol to cis,cis-muconic acid. Cells of strain SPG were able to degrade 2-nitrobenzoate in sterile as well as non-sterile soil microcosms. The results of microcosm studies showed that strain SPG degraded more than 90% of 2-nitrobenzoate within 10-12 days. This study clearly shows that Arthrobacter sp. SPG degraded 2-nitrobenzoate via a new pathway with formation of salicylate and catechol as metabolites. Arthrobacter sp. SPG may be used for bioremediation of 2-nitrobenzoate-contaminated sites due to its ability to degrade 2-nitrobenzoate in soil.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 24%
Researcher 5 20%
Student > Ph. D. Student 3 12%
Student > Doctoral Student 2 8%
Professor 1 4%
Other 3 12%
Unknown 5 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 36%
Biochemistry, Genetics and Molecular Biology 6 24%
Environmental Science 2 8%
Unspecified 1 4%
Earth and Planetary Sciences 1 4%
Other 0 0%
Unknown 6 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 May 2015.
All research outputs
#18,410,971
of 22,805,349 outputs
Outputs from Frontiers in Microbiology
#19,278
of 24,755 outputs
Outputs of similar age
#193,230
of 267,785 outputs
Outputs of similar age from Frontiers in Microbiology
#273
of 390 outputs
Altmetric has tracked 22,805,349 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,755 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,785 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 390 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.