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Fe biomineralization mirrors individual metabolic activity in a nitrate-dependent Fe(II)-oxidizer

Overview of attention for article published in Frontiers in Microbiology, September 2015
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Title
Fe biomineralization mirrors individual metabolic activity in a nitrate-dependent Fe(II)-oxidizer
Published in
Frontiers in Microbiology, September 2015
DOI 10.3389/fmicb.2015.00879
Pubmed ID
Authors

Jennyfer Miot, Laurent Remusat, Elodie Duprat, Adriana Gonzalez, Sylvain Pont, Mélanie Poinsot

Abstract

Microbial biomineralization sometimes leads to periplasmic encrustation, which is predicted to enhance microorganism preservation in the fossil record. Mineral precipitation within the periplasm is, however, thought to induce death, as a result of permeability loss preventing nutrient and waste transit across the cell wall. This hypothesis had, however, never been investigated down to the single cell level. Here, we cultured the nitrate reducing Fe(II) oxidizing bacteria Acidovorax sp. strain BoFeN1 that have been previously shown to promote the precipitation of a diversity of Fe minerals (lepidocrocite, goethite, Fe phosphate) encrusting the periplasm. We investigated the connection of Fe biomineralization with carbon assimilation at the single cell level, using a combination of electron microscopy and Nano-Secondary Ion Mass Spectrometry. Our analyses revealed strong individual heterogeneities of Fe biomineralization. Noteworthy, a small proportion of cells remaining free of any precipitate persisted even at advanced stages of biomineralization. Using pulse chase experiments with (13)C-acetate, we provide evidence of individual phenotypic heterogeneities of carbon assimilation, correlated with the level of Fe biomineralization. Whereas non- and moderately encrusted cells were able to assimilate acetate, higher levels of periplasmic encrustation prevented any carbon incorporation. Carbon assimilation only depended on the level of Fe encrustation and not on the nature of Fe minerals precipitated in the cell wall. Carbon assimilation decreased exponentially with increasing cell-associated Fe content. Persistence of a small proportion of non-mineralized and metabolically active cells might constitute a survival strategy in highly ferruginous environments. Eventually, our results suggest that periplasmic Fe biomineralization may provide a signature of individual metabolic status, which could be looked for in the fossil record and in modern environmental samples.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Unknown 43 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 27%
Student > Ph. D. Student 10 23%
Student > Bachelor 6 14%
Student > Master 4 9%
Student > Postgraduate 2 5%
Other 2 5%
Unknown 8 18%
Readers by discipline Count As %
Earth and Planetary Sciences 8 18%
Agricultural and Biological Sciences 8 18%
Environmental Science 7 16%
Chemistry 3 7%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Other 5 11%
Unknown 12 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2015.
All research outputs
#20,290,425
of 22,826,360 outputs
Outputs from Frontiers in Microbiology
#22,393
of 24,791 outputs
Outputs of similar age
#224,642
of 267,498 outputs
Outputs of similar age from Frontiers in Microbiology
#331
of 411 outputs
Altmetric has tracked 22,826,360 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,791 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 411 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.