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Mendeley readers
Attention Score in Context
| Title |
Comparison of methods for the enumeration of enterohemorrhagic Escherichia coli from veal hides and carcasses
|
|---|---|
| Published in |
Frontiers in Microbiology, September 2015
|
| DOI | 10.3389/fmicb.2015.01062 |
| Pubmed ID | |
| Authors |
Brandon E. Luedtke, Joseph M. Bosilevac |
| Abstract |
The increased association of enterohemorrhagic Escherichia coli (EHEC) with veal calves has led the United States Department of Agriculture Food Safety and Inspection Service to report results of veal meat contaminated with the Top 7 serogroups separately from beef cattle. However, detection methods that can also provide concentration for determining the prevalence and abundance of EHEC associated with veal are lacking. Here we compared the ability of qPCR and a molecular based most probable number assay (MPN) to detect and enumerate EHEC from veal hides at the abattoir and the resulting pre-intervention carcasses. In addition, digital PCR (dPCR) was used to analyze select samples. The qPCR assay was able to enumerate total EHEC in 32% of the hide samples with a range of approximately 34 to 91,412 CFUs/100 cm(2) (95% CI 4-113,460 CFUs/100 cm(2)). Using the MPN assay, total EHEC was enumerable in 48% of the hide samples and ranged from approximately 1 to greater than 17,022 CFUs/100 cm(2) (95% CI 0.4-72,000 CFUs/100 cm(2)). The carcass samples had lower amounts of EHEC with a range of approximately 4-275 CFUs/100 cm(2) (95% CI 3-953 CFUs/100 cm(2)) from 17% of samples with an enumerable amount of EHEC by qPCR. For the MPN assay, the carcass samples ranged from 0.1 to 1 CFUs/100 cm(2) (95% CI 0.02-4 CFUs/100 cm(2)) from 29% of the samples. The correlation coefficient between the qPCR and MPN enumeration methods indicated a moderate relation (R (2) = 0.39) for the hide samples while the carcass samples had no relation (R (2) = 0.002), which was likely due to most samples having an amount of total EHEC below the reliable limit of quantification for qPCR. Interestingly, after enrichment, 81% of the hide samples and 94% of the carcass samples had a detectable amount of total EHEC by qPCR. From our analysis, the MPN assay provided a higher percentage of enumerable hide and carcass samples, however determining an appropriate dilution range and the limited throughput offer additional challenges. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 23 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 26% |
| Researcher | 6 | 26% |
| Student > Ph. D. Student | 3 | 13% |
| Student > Bachelor | 2 | 9% |
| Lecturer | 1 | 4% |
| Other | 3 | 13% |
| Unknown | 2 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 22% |
| Engineering | 3 | 13% |
| Biochemistry, Genetics and Molecular Biology | 2 | 9% |
| Veterinary Science and Veterinary Medicine | 2 | 9% |
| Medicine and Dentistry | 2 | 9% |
| Other | 5 | 22% |
| Unknown | 4 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 September 2015.
All research outputs
#20,292,660
of 22,829,083 outputs
Outputs from Frontiers in Microbiology
#22,397
of 24,800 outputs
Outputs of similar age
#230,174
of 274,379 outputs
Outputs of similar age from Frontiers in Microbiology
#344
of 429 outputs
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So far Altmetric has tracked 24,800 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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