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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Single cell PCR amplification of diatoms using fresh and preserved samples
|
|---|---|
| Published in |
Frontiers in Microbiology, October 2015
|
| DOI | 10.3389/fmicb.2015.01084 |
| Pubmed ID | |
| Authors |
Paul B. Hamilton, Keely E. Lefebvre, Roger D. Bull |
| Abstract |
Single cell Chelex® DNA extraction and nested PCR amplification were used to examine partial gene sequences from natural diatom populations for taxonomic and phylogenetic studies at and above the level of species. DNA was extracted from cells that were either fresh collected or stored in RNAlater. Extractions from Lugol's fixation were also attempted with limited success. Three partial gene sequences (rbcL, 18S, and psbA) were recovered using existing and new primers with a nested or double nested PCR approach with amplification and success rates between 70 and 96%. An rbcL consensus tree grouped morphologically similar specimens and was consistent across the two primary sample treatments: fresh and RNAlater. This tool will greatly enhance the number of microscopic diatom taxa (and potentially other microbes) available for barcoding and phylogenetic studies. The near-term increase in sequence data for diatoms generated via routine single cell extractions and PCR will act as a multiproxy validation of longer-term next generation genomics. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 113 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | <1% |
| South Africa | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 110 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 22 | 19% |
| Student > Master | 17 | 15% |
| Researcher | 15 | 13% |
| Student > Bachelor | 14 | 12% |
| Student > Postgraduate | 7 | 6% |
| Other | 15 | 13% |
| Unknown | 23 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 46 | 41% |
| Environmental Science | 18 | 16% |
| Biochemistry, Genetics and Molecular Biology | 13 | 12% |
| Medicine and Dentistry | 2 | 2% |
| Earth and Planetary Sciences | 2 | 2% |
| Other | 5 | 4% |
| Unknown | 27 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 November 2015.
All research outputs
#18,429,163
of 22,830,751 outputs
Outputs from Frontiers in Microbiology
#19,314
of 24,801 outputs
Outputs of similar age
#200,908
of 279,403 outputs
Outputs of similar age from Frontiers in Microbiology
#313
of 440 outputs
Altmetric has tracked 22,830,751 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,801 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,403 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 440 others from the same source and published within six weeks on either side of this one. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.