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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Systemic colonization of clover (Trifolium repens) by Clostridium botulinum strain 2301
|
|---|---|
| Published in |
Frontiers in Microbiology, October 2015
|
| DOI | 10.3389/fmicb.2015.01207 |
| Pubmed ID | |
| Authors |
Matthias Zeiller, Michael Rothballer, Azuka N. Iwobi, Helge Böhnel, Frank Gessler, Anton Hartmann, Michael Schmid |
| Abstract |
In recent years, cases of botulism in cattle and other farm animals and also in farmers increased dramatically. It was proposed, that these cases could be affiliated with the spreading of compost or other organic manures contaminated with Clostridium botulinum spores on farm land. Thus, soils and fodder plants and finally farm animals could be contaminated. Therefore, the colonization behavior and interaction of the botulinum neurotoxin (BoNT D) producing C. botulinum strain 2301 and the non-toxin producing Clostridium sporogenes strain 1739 were investigated on clover (Trifolium repens) in a field experiment as well as in phytochamber experiments applying axenic and additionally soil based systems under controlled conditions. Plants were harvested and divided into root and shoot parts for further DNA isolation and polymerase chain reaction (PCR) assays; subsamples were fixed for fluorescence in situ hybridization analysis in combination with confocal laser scanning microscopy. In addition, we observed significant differences in the growth behavior of clover plants when inoculated with clostridial spores, indicating a plant growth promoting effect. Inoculated plants showed an increased growth index (shoot size, wet and dry weight) and an enlarged root system induced by the systemic colonization of clover by C. botulinum strain 2301. To target C. botulinum and C. sporogenes, 16S rDNA directed primers were used and to specifically detect C. botulinum, BoNT D toxin genes targeted primers, using a multiplex PCR approach, were applied. Our results demonstrate an effective colonization of roots and shoots of clover by C. botulinum strain 2301 and C. sporogenes strain 1739. Detailed analysis of colonization behavior showed that C. botulinum can occur as individual cells, in cell clusters and in microcolonies within the rhizosphere, lateral roots and within the roots tissue of clover. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 48 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 25% |
| Researcher | 7 | 15% |
| Student > Master | 6 | 13% |
| Student > Postgraduate | 3 | 6% |
| Student > Bachelor | 2 | 4% |
| Other | 6 | 13% |
| Unknown | 12 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 35% |
| Engineering | 4 | 8% |
| Biochemistry, Genetics and Molecular Biology | 4 | 8% |
| Environmental Science | 3 | 6% |
| Unspecified | 1 | 2% |
| Other | 4 | 8% |
| Unknown | 15 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 June 2022.
All research outputs
#6,426,442
of 22,831,537 outputs
Outputs from Frontiers in Microbiology
#6,568
of 24,806 outputs
Outputs of similar age
#81,442
of 284,235 outputs
Outputs of similar age from Frontiers in Microbiology
#100
of 425 outputs
Altmetric has tracked 22,831,537 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 24,806 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 284,235 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 425 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.