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Evaluation of Fungal Laccase Immobilized on Natural Nanostructured Bacterial Cellulose

Overview of attention for article published in Frontiers in Microbiology, November 2015
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Title
Evaluation of Fungal Laccase Immobilized on Natural Nanostructured Bacterial Cellulose
Published in
Frontiers in Microbiology, November 2015
DOI 10.3389/fmicb.2015.01245
Pubmed ID
Authors

Lin Chen, Min Zou, Feng F. Hong

Abstract

The aim of this work was to assess the possibility of using native bacterial nanocellulose (BC) as a carrier for laccase immobilization. BC was synthesized by Gluconacetobacter xylinus, which was statically cultivated in a mannitol-based medium and was freeze-dried to form BC sponge after purification. For the first time, fungal laccase from Trametes versicolor was immobilized on the native nanofibril network-structured BC sponge through physical adsorption and cross-linking with glutaraldehyde. The properties including morphologic and structural features of the BC as well as the immobilized enzyme were thoroughly investigated. It was found that enzyme immobilized by cross-linking exhibited broader pH operation range of high catalytic activity as well as higher running stability compared to free and adsorbed enzyme. Using ABTS as substrate, the optimum pH value was 3.5 for the adsorption-immobilized laccase and 4.0 for the crosslinking-immobilized laccase. The immobilized enzyme retained 69% of the original activity after being recycled seven times. Novel applications of the BC-immobilized enzyme tentatively include active packaging, construction of biosensors, and establishment of bioreactors.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 120 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 120 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 21 18%
Student > Master 14 12%
Student > Ph. D. Student 12 10%
Researcher 11 9%
Student > Doctoral Student 8 7%
Other 21 18%
Unknown 33 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 18 15%
Agricultural and Biological Sciences 16 13%
Chemistry 14 12%
Engineering 12 10%
Environmental Science 6 5%
Other 16 13%
Unknown 38 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 November 2015.
All research outputs
#20,295,501
of 22,832,057 outputs
Outputs from Frontiers in Microbiology
#22,407
of 24,810 outputs
Outputs of similar age
#236,863
of 282,783 outputs
Outputs of similar age from Frontiers in Microbiology
#360
of 440 outputs
Altmetric has tracked 22,832,057 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,810 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 440 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.