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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Construction of a Super-Competent Bacillus subtilis 168 Using the PmtlA-comKS Inducible Cassette
|
|---|---|
| Published in |
Frontiers in Microbiology, December 2015
|
| DOI | 10.3389/fmicb.2015.01431 |
| Pubmed ID | |
| Authors |
Regine Rahmer, Kambiz Morabbi Heravi, Josef Altenbuchner |
| Abstract |
Competence is a physiological state that enables Bacillus subtilis 168 to take up and internalize extracellular DNA. In practice, only a small subpopulation of B. subtilis 168 cells becomes competent when they enter stationary phase. In this study, we developed a new transformation method to improve the transformation efficiency of B. subtilis 168, specially in rich media. At first, different competence genes, namely comK, comS, and dprA, were alone or together integrated into the chromosome of B. subtilis 168 under control of mannitol-inducible P mtlA promoter. Overexpression of both comK and comS increased the transformation efficiency of B. subtilis REG19 with plasmid DNA by 6.7-fold compared to the wild type strain 168. This transformation efficiency reached its maximal level after 1.5 h of induction by mannitol. Besides, transformability of the REG19 cells was saturated in the presence of 100 ng dimeric plasmid or 3000 ng chromosomal DNA. Studying the influence of global regulators on the development of competence pointed out that important competence development factors, such as Spo0A, ComQXPA, and DegU, could be removed in REG19. On the other hand, efficient REG19 transformation remained highly dependent on the original copies of comK and comS regardless of the presence of P mtlA -comKS. Finally, novel plasmid-free strategies were used for transformation of REG19 based on Gibson assembly. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 1 | 33% |
| Switzerland | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 207 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Unknown | 206 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 46 | 22% |
| Researcher | 32 | 15% |
| Student > Master | 31 | 15% |
| Student > Bachelor | 27 | 13% |
| Student > Doctoral Student | 11 | 5% |
| Other | 18 | 9% |
| Unknown | 42 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 82 | 40% |
| Agricultural and Biological Sciences | 47 | 23% |
| Immunology and Microbiology | 11 | 5% |
| Chemistry | 5 | 2% |
| Engineering | 4 | 2% |
| Other | 11 | 5% |
| Unknown | 47 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 June 2022.
All research outputs
#2,934,005
of 22,764,165 outputs
Outputs from Frontiers in Microbiology
#2,671
of 24,656 outputs
Outputs of similar age
#52,160
of 389,145 outputs
Outputs of similar age from Frontiers in Microbiology
#47
of 405 outputs
Altmetric has tracked 22,764,165 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 24,656 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 389,145 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 405 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.