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Analysis of the First Temperate Broad Host Range Brucellaphage (BiPBO1) Isolated from B. inopinata

Overview of attention for article published in Frontiers in Microbiology, January 2016
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Title
Analysis of the First Temperate Broad Host Range Brucellaphage (BiPBO1) Isolated from B. inopinata
Published in
Frontiers in Microbiology, January 2016
DOI 10.3389/fmicb.2016.00024
Pubmed ID
Authors

Jens A. Hammerl, Cornelia Göllner, Sascha Al Dahouk, Karsten Nöckler, Jochen Reetz, Stefan Hertwig

Abstract

Brucella species are important human and animal pathogens. Though, only little is known about mobile genetic elements of these highly pathogenic bacteria. To date, neither plasmids nor temperate phages have been described in brucellae. We analyzed genomic sequences of various reference and type strains and identified a number of putative prophages residing within the Brucella chromosomes. By induction, phage BiPBO1 was isolated from Brucella inopinata. BiPBO1 is a siphovirus that infects several Brucella species including Brucella abortus and Brucella melitensis. Integration of the phage genome occurs adjacent to a tRNA gene in chromosome 1 (chr 1). The bacterial (attB) and phage (attP) attachment sites comprise an identical sequence of 46 bp. This sequence exists in many Brucella and Ochrobactrum species. The BiPBO1 genome is composed of a 46,877 bp double-stranded DNA. Eighty-seven putative gene products were determined, of which 32 could be functionally assigned. Strongest similarities were found to a temperate phage residing in the chromosome of Ochrobactrum anthropi ATCC 49188 and to prophages identified in several families belonging to the order rhizobiales. The data suggest that horizontal gene transfer may occur between Brucella and Ochrobactrum and underpin the close relationship of these environmental and pathogenic bacteria.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 36 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 22%
Student > Master 8 22%
Student > Ph. D. Student 5 14%
Professor 3 8%
Student > Bachelor 2 6%
Other 5 14%
Unknown 5 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 33%
Biochemistry, Genetics and Molecular Biology 5 14%
Immunology and Microbiology 4 11%
Environmental Science 2 6%
Veterinary Science and Veterinary Medicine 2 6%
Other 4 11%
Unknown 7 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 January 2016.
All research outputs
#20,879,072
of 23,498,099 outputs
Outputs from Frontiers in Microbiology
#23,507
of 25,939 outputs
Outputs of similar age
#336,479
of 399,925 outputs
Outputs of similar age from Frontiers in Microbiology
#436
of 488 outputs
Altmetric has tracked 23,498,099 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 25,939 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 488 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.