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Propagation and Characterization of Influenza Virus Stocks That Lack High Levels of Defective Viral Genomes and Hemagglutinin Mutations

Overview of attention for article published in Frontiers in Microbiology, March 2016
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  • Above-average Attention Score compared to outputs of the same age and source (51st percentile)

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4 X users

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127 Mendeley
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Title
Propagation and Characterization of Influenza Virus Stocks That Lack High Levels of Defective Viral Genomes and Hemagglutinin Mutations
Published in
Frontiers in Microbiology, March 2016
DOI 10.3389/fmicb.2016.00326
Pubmed ID
Authors

Jia Xue, Benjamin S. Chambers, Scott E. Hensley, Carolina B. López

Abstract

Influenza virus infections are responsible for more than 250,000 deaths annually. Influenza virus isolation, propagation, and characterization protocols are critical for completing reproducible basic research studies and for generating vaccine seed stocks. Detailed protocols for the isolation and identification of influenza virus have been recently reported (Eisfeld et al., 2014). However, there are few standardized protocols focused on the propagation and characterization of viral isolates, and as a result, viruses propagated in different conditions in different laboratories often have distinct in vitro and in vivo characteristics. Here, we focus on influenza A virus propagation and characterization in the laboratory taking into consideration the overall quality and composition of the virus stock to achieve consistency in virus yield, virulence, and immunostimulatory activity.

X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 127 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 127 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 28 22%
Researcher 22 17%
Student > Bachelor 11 9%
Student > Master 7 6%
Student > Postgraduate 6 5%
Other 18 14%
Unknown 35 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 28 22%
Immunology and Microbiology 24 19%
Agricultural and Biological Sciences 20 16%
Veterinary Science and Veterinary Medicine 6 5%
Pharmacology, Toxicology and Pharmaceutical Science 3 2%
Other 8 6%
Unknown 38 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 May 2016.
All research outputs
#15,344,393
of 25,757,133 outputs
Outputs from Frontiers in Microbiology
#11,883
of 29,780 outputs
Outputs of similar age
#158,763
of 315,722 outputs
Outputs of similar age from Frontiers in Microbiology
#264
of 554 outputs
Altmetric has tracked 25,757,133 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 29,780 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 315,722 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 554 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.