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Isolation of Oxamyl-degrading Bacteria and Identification of cehA as a Novel Oxamyl Hydrolase Gene

Overview of attention for article published in Frontiers in Microbiology, April 2016
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Title
Isolation of Oxamyl-degrading Bacteria and Identification of cehA as a Novel Oxamyl Hydrolase Gene
Published in
Frontiers in Microbiology, April 2016
DOI 10.3389/fmicb.2016.00616
Pubmed ID
Authors

Konstantina Rousidou, Eleni Chanika, Dafne Georgiadou, Eftychia Soueref, Demetra Katsarou, Panagiotis Kolovos, Spyridon Ntougias, Maria Tourna, Emmanuel A. Tzortzakakis, Dimitrios G. Karpouzas

Abstract

Microbial degradation is the main process controlling the environmental dissipation of the nematicide oxamyl. Despite that, little is known regarding the microorganisms involved in its biotransformation. We report the isolation of four oxamyl-degrading bacterial strains from an agricultural soil exhibiting enhanced biodegradation of oxamyl. Multilocus sequence analysis (MLSA) assigned the isolated bacteria to different subgroups of the genus Pseudomonas. The isolated bacteria hydrolyzed oxamyl to oxamyl oxime, which was not further transformed, and utilized methylamine as a C and N source. This was further supported by the detection of methylamine dehydrogenase in three of the four isolates. All oxamyl-degrading strains carried a gene highly homologous to a carbamate-hydrolase gene cehA previously identified in carbaryl- and carbofuran-degrading strains. Transcription analysis verified its direct involvement in the hydrolysis of oxamyl. Selected isolates exhibited relaxed degrading specificity and transformed all carbamates tested including the oximino carbamates aldicarb and methomyl (structurally related to oxamyl) and the aryl-methyl carbamates carbofuran and carbaryl which share with oxamyl only the carbamate moiety.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 2%
Unknown 53 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 19%
Student > Master 9 17%
Researcher 7 13%
Student > Bachelor 3 6%
Professor 2 4%
Other 4 7%
Unknown 19 35%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 22%
Biochemistry, Genetics and Molecular Biology 8 15%
Environmental Science 5 9%
Chemical Engineering 2 4%
Business, Management and Accounting 1 2%
Other 2 4%
Unknown 24 44%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 May 2016.
All research outputs
#18,455,405
of 22,867,327 outputs
Outputs from Frontiers in Microbiology
#19,371
of 24,877 outputs
Outputs of similar age
#218,961
of 299,065 outputs
Outputs of similar age from Frontiers in Microbiology
#418
of 567 outputs
Altmetric has tracked 22,867,327 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,877 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 299,065 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 567 others from the same source and published within six weeks on either side of this one. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.