Title |
Current Technological Improvements in Enzymes toward Their Biotechnological Applications
|
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Published in |
Frontiers in Microbiology, June 2016
|
DOI | 10.3389/fmicb.2016.00965 |
Pubmed ID | |
Authors |
Mehak Baweja, Lata Nain, Yutaka Kawarabayasi, Pratyoosh Shukla |
Abstract |
Enzymes from extremophiles are creating interest among researchers due to their unique properties and the enormous power of catalysis at extreme conditions. Since community demands are getting more intensified, therefore, researchers are applying various approaches viz. metagenomics to increase the database of extremophilic species. Furthermore, the innovations are being made in the naturally occurring enzymes utilizing various tools of recombinant DNA technology and protein engineering, which allows redesigning of the enzymes for its better fitment into the process. In this review, we discuss the biochemical constraints of psychrophiles during survival at the lower temperature. We summarize the current knowledge about the sources of such enzymes and their in vitro modification through mutagenesis to explore their biotechnological potential. Finally, we recap the microbial cell surface display to enhance the efficiency of the process in cost effective way. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Switzerland | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Brazil | 2 | 2% |
Japan | 1 | <1% |
Unknown | 121 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 21 | 17% |
Researcher | 18 | 15% |
Student > Master | 17 | 14% |
Student > Ph. D. Student | 12 | 10% |
Student > Doctoral Student | 6 | 5% |
Other | 20 | 16% |
Unknown | 30 | 24% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 38 | 31% |
Agricultural and Biological Sciences | 27 | 22% |
Environmental Science | 7 | 6% |
Immunology and Microbiology | 5 | 4% |
Engineering | 3 | 2% |
Other | 9 | 7% |
Unknown | 35 | 28% |