Title |
Anti-biofilm Activities from Resveratrol against Fusobacterium nucleatum
|
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Published in |
Frontiers in Microbiology, July 2016
|
DOI | 10.3389/fmicb.2016.01065 |
Pubmed ID | |
Authors |
Zhiyan He, Zhengwei Huang, Wei Zhou, Zisheng Tang, Rui Ma, Jingping Liang |
Abstract |
Fusobacterium nucleatum is a Gram-negative, anaerobic bacterium that plays an important role in dental plaque biofilm formation. In this study, we evaluate the effect of resveratrol, a phytoalexin compound, on F. nucleatum biofilm formation. The effects of different concentrations of resveratrol on biofilms formed on 96-well microtiter plates at different time points were determined by the MTT assay. The structures and thicknesses of the biofilm were observed by confocal laser scanning microscopy (CLSM), and gene expression was investigated by real-time PCR. The results showed that resveratrol at sub-MIC levels can significantly decrease biofilm formation, whereas it does not affect the bacterial growth rate. It was observed by CLSM images that the biofilm was visually decreased with increasing concentrations of resveratrol. Gene expression was down regulated in the biofilm in the presence of resveratrol. Our results revealed that resveratrol can effectively inhibit biofilm formation. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 33% |
Switzerland | 1 | 17% |
Unknown | 3 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 50% |
Scientists | 3 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 51 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 9 | 18% |
Student > Bachelor | 5 | 10% |
Student > Doctoral Student | 4 | 8% |
Student > Master | 4 | 8% |
Other | 2 | 4% |
Other | 6 | 12% |
Unknown | 21 | 41% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 16% |
Medicine and Dentistry | 7 | 14% |
Immunology and Microbiology | 6 | 12% |
Agricultural and Biological Sciences | 4 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 6% |
Other | 2 | 4% |
Unknown | 21 | 41% |