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Attention Score in Context
| Title |
A Flow Cytometric and Computational Approaches to Carbapenems Affinity to the Different Types of Carbapenemases
|
|---|---|
| Published in |
Frontiers in Microbiology, August 2016
|
| DOI | 10.3389/fmicb.2016.01259 |
| Pubmed ID | |
| Authors |
Cidália Pina-Vaz, Ana P. Silva, Isabel Faria-Ramos, Rita Teixeira-Santos, Daniel Moura, Tatiana F. Vieira, Sérgio F. Sousa, Sofia Costa-de-Oliveira, Rafael Cantón, Acácio G. Rodrigues |
| Abstract |
The synergy of carbapenem combinations regarding Enterobacteriaceae producing different types of carbapenemases was study through different approaches: flow cytometry and computational analysis. Ten well characterized Enterobacteriaceae (KPC, verona integron-encoded metallo-β-lactamases -VIM and OXA-48-like enzymes) were selected for the study. The cells were incubated with a combination of ertapenem with imipenem, meropenem, or doripenem and killing kinetic curves performed with and without reinforcements of the drugs. A cephalosporin was also used in combination with ertapenem. A flow cytometric assay with DiBAC4-(3), a membrane potential dye, was developed in order to evaluate the cellular lesion after 2 h incubation. A chemical computational study was performed to understand the affinity of the different drugs to the different types of enzymes. Flow cytometric analysis and time-kill assays showed a synergic effect against KPC and OXA-48 producing-bacteria with all combinations; only ertapenem with imipenem was synergic against VIM producing-bacteria. A bactericidal effect was observed in OXA-48-like enzymes. Ceftazidime plus ertapenem was synergic against ESBL-negative KPC producing-bacteria. Ertapenem had the highest affinity for those enzymes according to chemical computational study. The synergic effect between ertapenem and others carbapenems against different carbapenemase-producing bacteria, representing a therapeutic choice, was described for the first time. Easier and faster laboratorial methods for carbapenemase characterization are urgently needed. The design of an ertapenem derivative with similar affinity to carbapenemases but exhibiting more stable bonds was demonstrated as highly desirable. |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 19% |
| Student > Ph. D. Student | 5 | 16% |
| Student > Doctoral Student | 3 | 9% |
| Other | 2 | 6% |
| Researcher | 2 | 6% |
| Other | 5 | 16% |
| Unknown | 9 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 22% |
| Chemistry | 3 | 9% |
| Medicine and Dentistry | 3 | 9% |
| Agricultural and Biological Sciences | 2 | 6% |
| Environmental Science | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 14 | 44% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2016.
All research outputs
#20,338,537
of 22,884,315 outputs
Outputs from Frontiers in Microbiology
#22,520
of 24,921 outputs
Outputs of similar age
#317,038
of 361,769 outputs
Outputs of similar age from Frontiers in Microbiology
#369
of 433 outputs
Altmetric has tracked 22,884,315 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,921 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 361,769 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 433 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.