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A Screening Method for the Isolation of Polyhydroxyalkanoate-Producing Purple Non-sulfur Photosynthetic Bacteria from Natural Seawater

Overview of attention for article published in Frontiers in Microbiology, September 2016
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Title
A Screening Method for the Isolation of Polyhydroxyalkanoate-Producing Purple Non-sulfur Photosynthetic Bacteria from Natural Seawater
Published in
Frontiers in Microbiology, September 2016
DOI 10.3389/fmicb.2016.01509
Pubmed ID
Authors

Mieko Higuchi-Takeuchi, Kumiko Morisaki, Keiji Numata

Abstract

Polyhydroxyalkanoates (PHAs) are a family of biopolyesters accumulated by a variety of microorganisms as carbon and energy storage under starvation conditions. We focused on marine purple non-sulfur photosynthetic bacteria as host microorganisms for PHA production and developed a method for their isolation from natural seawater. To identify novel PHA-producing marine purple non-sulfur photosynthetic bacteria, natural seawaters were cultured in nutrient-rich medium for purple non-sulfur photosynthetic bacteria, and twelve pink- or red-pigmented colonies were picked up. Gas chromatography mass spectrometry analysis revealed that four isolates synthesized PHA at levels ranging from 0.5 to 24.4 wt% of cell dry weight. The 16S ribosomal RNA sequence analysis revealed that one isolate (HM2) showed 100% identity to marine purple non-sulfur photosynthetic bacteria. In conclusion, we have demonstrated in this study that PHA-producing marine purple non-sulfur photosynthetic bacteria can be isolated from natural seawater under nutrient-rich conditions.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 119 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 <1%
Unknown 118 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 23 19%
Student > Ph. D. Student 21 18%
Student > Master 14 12%
Researcher 11 9%
Student > Doctoral Student 6 5%
Other 13 11%
Unknown 31 26%
Readers by discipline Count As %
Agricultural and Biological Sciences 26 22%
Biochemistry, Genetics and Molecular Biology 22 18%
Environmental Science 10 8%
Engineering 9 8%
Immunology and Microbiology 6 5%
Other 8 7%
Unknown 38 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 September 2016.
All research outputs
#20,342,896
of 22,889,074 outputs
Outputs from Frontiers in Microbiology
#22,527
of 24,933 outputs
Outputs of similar age
#278,296
of 320,659 outputs
Outputs of similar age from Frontiers in Microbiology
#342
of 431 outputs
Altmetric has tracked 22,889,074 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 24,933 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 320,659 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 431 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.