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In vitro Determination of Extracellular Proteins from Xylella fastidiosa

Overview of attention for article published in Frontiers in Microbiology, December 2016
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Title
In vitro Determination of Extracellular Proteins from Xylella fastidiosa
Published in
Frontiers in Microbiology, December 2016
DOI 10.3389/fmicb.2016.02090
Pubmed ID
Authors

Juliano S. Mendes, André S. Santiago, Marcelo A. S. Toledo, Maria A. C. Horta, Alessandra A. de Souza, Ljubica Tasic, Anete P. de Souza

Abstract

The phytopathogen Xylella fastidiosa causes economic losses in important agricultural crops. Xylem vessel occlusion caused by biofilm formation is the major mechanism underlying the pathogenicity of distinct strains of X. fastidiosa. Here, we provide a detailed in vitro characterization of the extracellular proteins of X. fastidiosa. Based on the results, we performed a comparison with a strain J1a12, which cannot induce citrus variegated chlorosis symptoms when inoculated into citrus plants. We then extend this approach to analyze the extracellular proteins of X. fastidiosa in media supplemented with calcium. We verified increases in extracellular proteins concomitant with the days of growth and, consequently, biofilm development (3-30 days). Outer membrane vesicles carrying toxins were identified beginning at 10 days of growth in the 9a5c strain. In addition, a decrease in extracellular proteins in media supplemented with calcium was observed in both strains. Using mass spectrometry, 71 different proteins were identified during 30 days of X. fastidiosa biofilm development, including proteases, quorum-sensing proteins, biofilm formation proteins, hypothetical proteins, phage-related proteins, chaperones, toxins, antitoxins, and extracellular vesicle membrane components.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 74 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 14 19%
Student > Ph. D. Student 11 15%
Student > Bachelor 8 11%
Student > Master 7 9%
Professor 5 7%
Other 14 19%
Unknown 15 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 25 34%
Biochemistry, Genetics and Molecular Biology 10 14%
Chemistry 4 5%
Medicine and Dentistry 4 5%
Environmental Science 3 4%
Other 5 7%
Unknown 23 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 May 2018.
All research outputs
#16,870,624
of 24,805,946 outputs
Outputs from Frontiers in Microbiology
#17,432
of 28,290 outputs
Outputs of similar age
#267,820
of 430,901 outputs
Outputs of similar age from Frontiers in Microbiology
#280
of 400 outputs
Altmetric has tracked 24,805,946 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 28,290 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
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We're also able to compare this research output to 400 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.