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Attention Score in Context
| Title |
Detection and Physicochemical Characterization of Membrane Vesicles (MVs) of Lactobacillus reuteri DSM 17938
|
|---|---|
| Published in |
Frontiers in Microbiology, June 2017
|
| DOI | 10.3389/fmicb.2017.01040 |
| Pubmed ID | |
| Authors |
Rossella Grande, Christian Celia, Gabriella Mincione, Annarita Stringaro, Luisa Di Marzio, Marisa Colone, Maria C. Di Marcantonio, Luca Savino, Valentina Puca, Roberto Santoliquido, Marcello Locatelli, Raffaella Muraro, Luanne Hall-Stoodley, Paul Stoodley |
| Abstract |
Membrane vesicles (MVs) are bilayer structures which bleb from bacteria, and are important in trafficking biomolecules to other bacteria or host cells. There are few data about MVs produced by the Gram-positive commensal-derived probiotic Lactobacillus reuteri; however, MVs from this species may have potential therapeutic benefit. The aim of this study was to detect and characterize MVs produced from biofilm (bMVs), and planktonic (pMVs) phenotypes of L. reuteri DSM 17938. MVs were analyzed for structure and physicochemical characterization by Scanning Electron Microscope (SEM) and Dynamic Light Scattering (DLS). Their composition was interrogated using various digestive enzyme treatments and subsequent Transmission Electron Microscopy (TEM) analysis. eDNA (extracellular DNA) was detected and quantified using PicoGreen. We found that planktonic and biofilm of L. reuteri cultures generated MVs with a broad size distribution. Our data also showed that eDNA was associated with pMVs and bMVs (eMVsDNA). DNase I treatment demonstrated no modifications of MVs, suggesting that an eDNA-MVs complex protected the eMVsDNA. Proteinase K and Phospholipase C treatments modified the structure of MVs, showing that lipids and proteins are important structural components of L. reuteri MVs. The biological composition and the physicochemical characterization of MVs generated by the probiotic L. reuteri may represent a starting point for future applications in the development of vesicles-based therapeutic systems. |
Mendeley readers
The data shown below were compiled from readership statistics for 153 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 153 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 24 | 16% |
| Student > Ph. D. Student | 22 | 14% |
| Student > Master | 14 | 9% |
| Student > Bachelor | 12 | 8% |
| Student > Doctoral Student | 11 | 7% |
| Other | 30 | 20% |
| Unknown | 40 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 33 | 22% |
| Agricultural and Biological Sciences | 23 | 15% |
| Immunology and Microbiology | 22 | 14% |
| Medicine and Dentistry | 6 | 4% |
| Chemistry | 5 | 3% |
| Other | 13 | 8% |
| Unknown | 51 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 May 2017.
All research outputs
#5,795,772
of 22,974,684 outputs
Outputs from Frontiers in Microbiology
#5,508
of 25,034 outputs
Outputs of similar age
#92,360
of 317,512 outputs
Outputs of similar age from Frontiers in Microbiology
#194
of 529 outputs
Altmetric has tracked 22,974,684 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 25,034 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 317,512 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 529 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.