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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Cellular Response to Ciprofloxacin in Low-Level Quinolone-Resistant Escherichia coli
|
|---|---|
| Published in |
Frontiers in Microbiology, July 2017
|
| DOI | 10.3389/fmicb.2017.01370 |
| Pubmed ID | |
| Authors |
Jesús Machuca, Esther Recacha, Alejandra Briales, Paula Díaz-de-Alba, Jesús Blazquez, Álvaro Pascual, José-Manuel Rodríguez-Martínez |
| Abstract |
Bactericidal activity of quinolones has been related to a combination of DNA fragmentation, reactive oxygen species (ROS) production and programmed cell death (PCD) systems. The underlying molecular systems responsible for reducing bactericidal effect during antimicrobial therapy in low-level quinolone resistance (LLQR) phenotypes need to be clarified. To do this and also define possible new antimicrobial targets, the transcriptome profile of isogenic Escherichia coli harboring quinolone resistance mechanisms in the presence of a clinical relevant concentration of ciprofloxacin was evaluated. A marked differential response to ciprofloxacin of either up- or downregulation was observed in LLQR strains. Multiple genes implicated in ROS modulation (related to the TCA cycle, aerobic respiration and detoxification systems) were upregulated (sdhC up to 63.5-fold) in mutants with LLQR. SOS system components were downregulated (recA up to 30.7-fold). yihE, a protective kinase coding for PCD, was also upregulated (up to 5.2-fold). SdhC inhibition sensitized LLQR phenotypes (up to ΔLog = 2.3 after 24 h). At clinically relevant concentrations of ciprofloxacin, gene expression patterns in critical systems to bacterial survival and mutant development were significantly modified in LLQR phenotypes. Chemical inhibition of SdhC (succinate dehydrogenase) validated modulation of ROS as an interesting target for bacterial sensitization. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 47 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 17% |
| Student > Ph. D. Student | 7 | 15% |
| Student > Bachelor | 5 | 11% |
| Student > Master | 5 | 11% |
| Student > Doctoral Student | 4 | 9% |
| Other | 9 | 19% |
| Unknown | 9 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 14 | 30% |
| Medicine and Dentistry | 5 | 11% |
| Agricultural and Biological Sciences | 4 | 9% |
| Immunology and Microbiology | 3 | 6% |
| Nursing and Health Professions | 2 | 4% |
| Other | 7 | 15% |
| Unknown | 12 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 August 2017.
All research outputs
#20,441,465
of 22,996,001 outputs
Outputs from Frontiers in Microbiology
#22,658
of 25,075 outputs
Outputs of similar age
#274,954
of 315,218 outputs
Outputs of similar age from Frontiers in Microbiology
#466
of 534 outputs
Altmetric has tracked 22,996,001 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 25,075 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 315,218 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 534 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.