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Mendeley readers
| Title |
Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum
|
|---|---|
| Published in |
Frontiers in Microbiology, August 2017
|
| DOI | 10.3389/fmicb.2017.01647 |
| Pubmed ID | |
| Authors |
Baoling Li, Haizhen Wu, Na Li, Jiayuan Su, Ruilian Jia, Jianlin Jiang, Yaoyu Feng, Lihua Xiao |
| Abstract |
Cryptosporidium spp. are important causes of diarrhea in humans, ruminants, and other mammals. Comparative genomic analysis indicated that genetically related and host-adapted Cryptosporidium species have different numbers of subtelomeric genes encoding the Cryptosporidium-specific MEDLE family of secreted proteins, which could contribute to differences in host specificity. In this study, a Cryptosporidium parvum-specific member of the protein family MEDLE-2 encoded by cgd5_4590 was cloned and expressed in Escherichia coli. Immunofluorescent staining with antibodies generated from the recombinant protein showed the expression of the protein in sporozoites and development stages. In vitro neutralization assay with the antibodies partially blocked the invasion of sporozoites. These results support the potential involvement of MEDLE-2 in the invasion of host cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 31% |
| Researcher | 5 | 31% |
| Professor > Associate Professor | 1 | 6% |
| Student > Master | 1 | 6% |
| Unknown | 4 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 31% |
| Biochemistry, Genetics and Molecular Biology | 3 | 19% |
| Immunology and Microbiology | 2 | 13% |
| Medicine and Dentistry | 2 | 13% |
| Unknown | 4 | 25% |